1.14.18.9: 4alpha-methylsterol monooxygenase

This is an abbreviated version!
For detailed information about 4alpha-methylsterol monooxygenase, go to the full flat file.

Reaction

Synonyms

4,4-dimethyl-sterol 4alpha-methyl-oxidase, 4,4-dimethyl-zymosterol 4alpha-methyl-oxidase, 4-methyl sterol oxidase, 4-methylsterol oxidase, C-4 methyl sterol oxidase, C-4 sterol methyl oxidase, EC 1.14.13.72, EC 1.14.99.16, ERG25, ERG25p, GintSMO protein, methyl sterol oxidase, methylsterol hydroxylase, methylsterol monooxygenase, NAD(P)H-dependent methylsterol oxidase, SC4MOL, SMO, sterol C-4 methyloxidase, sterol C4 methyl oxidase, sterol-C4-methyl oxidase, sterol-C4-methyl-oxidase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.18.9 4alpha-methylsterol monooxygenase

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112	AA Sequence
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62	Disease/ Diagnostics
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1	Ki Value [mM]
2	KM Value [mM]
18	Localization
1	Metals/Ions
3	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
182	Organism
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28	Substrates and Products (Substrate)
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37	Synonyms
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Purification

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Purification on EC 1.14.18.9 - 4alpha-methylsterol monooxygenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

DEAE-cellulose column chromatography

Rattus norvegicus

-

742817

extraradial mycelium collected from solubilized medium in buffer (50 mM Tris-HCl, pH 7.5, 10 mM EDTA), vacuum filtrated, frozen in liquid nitrogen

Rhizophagus intraradices

C5HVZ4

698075

gel filtration, ammonium sulfate fractionation, affinity chromatography

Rattus norvegicus

-

285357

polyethylene glycol fractionation, DEAE-Sephacel gel filtration, CM-Sepharose column chromatography, and cytochrome b5-Sepharose column chromatography

Rattus norvegicus

-

742818