1.14.18.9: 4alpha-methylsterol monooxygenase

This is an abbreviated version!
For detailed information about 4alpha-methylsterol monooxygenase, go to the full flat file.

Reaction

Synonyms

4,4-dimethyl-sterol 4alpha-methyl-oxidase, 4,4-dimethyl-zymosterol 4alpha-methyl-oxidase, 4-methyl sterol oxidase, 4-methylsterol oxidase, C-4 methyl sterol oxidase, C-4 sterol methyl oxidase, EC 1.14.13.72, EC 1.14.99.16, ERG25, ERG25p, GintSMO protein, methyl sterol oxidase, methylsterol hydroxylase, methylsterol monooxygenase, NAD(P)H-dependent methylsterol oxidase, SC4MOL, SMO, sterol C-4 methyloxidase, sterol C4 methyl oxidase, sterol-C4-methyl oxidase, sterol-C4-methyl-oxidase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.18.9 4alpha-methylsterol monooxygenase

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112	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
10	Cofactor
62	Disease/ Diagnostics
4	General Information
8	Inhibitors
1	Ki Value [mM]
2	KM Value [mM]
18	Localization
1	Metals/Ions
3	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
182	Organism
9	Pathway
2	pI Value
3	Posttranslational Modification
7	Protein Variants
4	Purification (Commentary)
7	Reaction
3	Reaction Type
19	Reference
12	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
28	Substrates and Products (Substrate)
8	Subunits
37	Synonyms
1	Systematic Name

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Cloned on EC 1.14.18.9 - 4alpha-methylsterol monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA and amino acid sequence determination and analysis of wild-type and mutant strains

Saccharomyces cerevisiae

-

658997

DNA is extracted from extraradial mycelium growing around Cichorium intybus, PCR-amplification, cloned into pGEM-T Vector System for sequencing, for complementation assay in Saccharomyces cerevisiae mutant erg25-25c (methyl oxidase deficient) the GintSMO gene is amplified and cloned into the NotI-linearized yeast expression vector pFL61

Rhizophagus intraradices

C5HVZ4

698075

gene Pcerg25A, DNA and amino acid sequence determination and analysis, Pcerg25A is located in the DNA region containing the penicillin gene cluster, and thus its copy number is dependent on the patterns of the cluster region, up to eight copies of Pcerg25A are found in the high-productivity strain NCPC 10086, functional complementation of the erg25-deficient Saccharomyces cerevisiae strain ATCC 4024690, subcloning in Escherichia coli strain DH5alpha, phylogenetic analysis, overview

Penicillium chrysogenum

B5ST90, B5T004

686813

gene Pcerg25B, DNA and amino acid sequence determination and analysis, Pcerg25B is present in just a single copy in Penicillium chrysogenum genomes, functional complementation of the erg25-deficient Saccharomyces cerevisiae strain ATCC 4024690, subcloning in Escherichia coli strain DH5alpha, phylogenetic analysis, overview

Penicillium chrysogenum

B5ST90, B5T004

686813