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Ac4-5S-GlcNAc + [protein]-L-serine
UDP + ?
the donor substrate analogues Ac4-5S-GlcNAc and benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, might reduce the flux through the hexosamine pathway and reduce the amount of intracellular UDP-GlcNAc with potential side effects on glycan synthesis
-
-
?
adhesin PsrP + UDP-GlcNAc
? + UDP
benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside + [protein]-L-serine
UDP + ?
the donor substrate analogues Ac4-5S-GlcNAc and benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside, might reduce the flux through the hexosamine pathway and reduce the amount of intracellular UDP-GlcNAc with potential side effects on glycan synthesis
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
casein kinase II + UDP-GlcNAc
? + UDP
-
-
-
-
?
casein kinase II + UDP-N-azidoacetylglucosamine
? + UDP
-
-
-
-
?
casein kinase II peptide + UDP-GlcNAc
? + UDP
-
-
-
-
?
casein kinase II peptide + UDP-GlcNAc
UDP + ?
-
much poorer substrate than Nup 62
-
-
?
crystalline alpha + UDP-GlcNAc
? + UDP
-
i.e. small heat-shock protein crystalline alpha
-
-
?
GSK-3beta + UDP-GlcNAc
UDP + ?
-
rabbit skeletal muscle glycogen synthase kinase (GSK) -3beta
-
-
?
KENSPCVTPVSTA + UDP-GlcNAc
? + UDP
-
-
-
?
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GalNAc
? + UDP
-
-
-
?
KKKYPGGSTPVSSANMM + UDP-4-deoxy-GlcNAc
? + UDP
22.2% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GalNAc
? + UDP
37.7% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-6-deoxy-GlcNAc
? + UDP
85% yield
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAc
? + UDP
peptide acceptor derived from casein kinase II
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAc
UDP + ?
-
Pep-CKII, known natural substrate for OGT
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNAz
? + UDP
peptide acceptor derived from casein kinase II
-
-
?
KKKYPGGSTPVSSANMM + UDP-GlcNPr
? + UDP
the close vicinity between Met501 and the N-acyl group of GlcNPr, as well as the hydrophobic environment near Met501, are responsible for the selective binding of UDP-GlcNPr
-
-
?
mitochondrial motor-adaptor protein milton + UDP-GlcNAc
? + UDP
-
-
mitochondrial motor-adaptor protein Milton is a required substrate for OGT to arrest mitochondrial motility by mapping and mutating the key O-GlcNAcylated serine residues
-
?
nucleoporin p62 + UDP-GlcNAc
? + UDP
-
high affinity substrate
-
-
?
nucleoporin p62 + UDP-N-azidoacetylglucosamine
? + UDP
-
-
-
?
OIP106 protein + UDP-GlcNAc
O-GlcNAc-OIP106 protein + UDP
-
N-terminal deletions of OIP106 are generated as S-tagged constructs: DELTAnCC, DELTA491, DELTA639, DELTA859
-
-
?
RBL-2 + UDP-GlcNAc
? + UDP
acceptor RBL-2 is a key regulator of entry into cell division. Residue Ser420 is a possible O-GlcNAc site in RBL-2. Substitution of Ser 420 inhibits OGT activity
-
-
?
UDP-GlcNAc + Abi2 protein
UDP + N-acetly-D-glucosaminyl-[Abi2 protein]
-
-
-
?
UDP-GlcNAc + Abl2 protein
UDP + N-acetly-D-glucosaminyl-[Ab12 protein]
-
-
-
?
UDP-GlcNAc + Ablim2 protein
UDP + N-acetly-D-glucosaminyl-[Ablim2 protein]
-
-
-
?
UDP-GlcNAc + Add1 protein
UDP + N-acetly-D-glucosaminyl-[Add1 protein]
-
-
-
?
UDP-GlcNAc + AIPVSREEK
UDP + AIPV-(GlcNAc)SREEK
-
-
-
-
?
UDP-GlcNAc + Amot protein
UDP + N-acetly-D-glucosaminyl-[Amot protein]
-
-
-
?
UDP-GlcNAc + Arhgap32 protein
UDP + N-acetly-D-glucosaminyl-[Arhgap32 protein]
-
-
-
?
UDP-GlcNAc + Atf2 protein
UDP + N-acetly-D-glucosaminyl-[Atf2 protein]
-
-
-
?
UDP-GlcNAc + beta-amyloid associated protein
GlcNAc-beta-amyloid associated protein + UDP
-
-
-
-
?
UDP-GlcNAc + c-MYC intron binding protein 1
UDP + N-acetyl-D-gluosaminyl-[c-MYC intron binding protein 1]
-
-
-
?
UDP-GlcNAc + calcium/calmodulin-dependent kinase IV
UDP + N-acetyl-D-glucosaminyl-[calcium/calmodulin-dependent kinase IV]
-
-
-
?
UDP-GlcNAc + CARM1 protein
UDP + N-acetyl-D-glucosaminyl-[CARM1 protein]
-
-
-
?
UDP-GlcNAc + CKII peptide
UDP + N-acetyl-D-glucosaminyl-[CKII peptide]
UDP-GlcNAc + CKII peptide
UDP + N-acetylglucosaminyl-CKII alpha-peptide
PGGSTPVSSANMM
-
-
?
UDP-GlcNAc + Clasp2 protein
UDP + N-acetly-D-glucosaminyl-[Clasp2 protein]
-
-
-
?
UDP-GlcNAc + Clip1 protein
UDP + N-acetly-D-glucosaminyl-[Clip1 protein]
-
-
-
?
UDP-GlcNAc + Cnot4 protein
UDP + N-acetly-D-glucosaminyl-[Cnot4 protein]
-
-
-
?
UDP-GlcNAc + Cnskr2 protein
UDP + N-acetly-D-glucosaminyl-[Cnskr2 protein]
-
-
-
?
UDP-GlcNAc + Corp1b protein
UDP + N-acetly-D-glucosaminyl-[Corp1b protein]
-
-
-
?
UDP-GlcNAc + Crtc1 protein
UDP + N-acetly-D-glucosaminyl-[Crtc1 protein]
-
-
-
?
UDP-GlcNAc + CSNK1D
CSNK1D-GlcNAc + UDP
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + DCTN1
DCTN1-GlcNAc + UDP
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + DELTA639 protein
UDP + N-acetyl-D-glucosaminyl-[DELTA639 protein]
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-GlcNAc + DELTAnCC protein
UDP + N-acetyl-D-glucosaminyl-[DELTAnCC protein]
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-GlcNAc + Dlgap2 protein
UDP + N-acetly-D-glucosaminyl-[Dlgap2 protein]
-
-
-
?
UDP-GlcNAc + Dvl1 protein
UDP + N-acetly-D-glucosaminyl-[Dv11 protein]
-
-
-
?
UDP-GlcNAc + dynamin-related protein 1
UDP + dynamin-related protein 1-GlcNAc
dynamin-related protein 1 is O-linked-N-acetyl-glucosamine-glycosylated at threonine 585 and 586
-
-
?
UDP-GlcNAc + Enah protein
UDP + N-acetly-D-glucosaminyl-[Enah protein]
-
-
-
?
UDP-GlcNAc + Foxk2 protein
UDP + N-acetly-D-glucosaminyl-[Foxk2 protein]
-
-
-
?
UDP-GlcNAc + Foxp1 protein
UDP + N-acetly-D-glucosaminyl-[Foxp1 protein]
-
-
-
?
UDP-GlcNAc + Frs3 protein
UDP + N-acetly-D-glucosaminyl-[Frs3 protein]
-
-
-
?
UDP-GlcNAc + glutathione S-transferase
glutathione S-transferase-GlcNAc + UDP
UDP-GlcNAc + glutathione S-transferase-CARM1
glutathione S-transferase-CARM1-GlcNAc + UDP
UDP-GlcNAc + glutathione S-transferase-MYPT1
glutathione S-transferase-MYPT1-GlcNAc + UDP
UDP-GlcNAc + H2O
UDP + GlcNAc
UDP-GlcNAc + host cell factor C1
UDP + N-acetyl-D-gluosaminyl-[host cell factor C1]
the enzyme both O-GlcNAcylates the HCF-1N subunit and directly cleaves the host cell factor-1PRO repeat
-
-
?
UDP-GlcNAc + ITISETPSSTTTTQITK
UDP + ITI-(GlcNAc)SETPSSTTTTQITK
-
-
-
-
?
UDP-GlcNAc + KKFELLPTPPLSPSRR
UDP + KKFELLP-(GlcNAc)TPPLSPSRR
-
-
-
-
?
UDP-GlcNAc + Mamld1 protein
UDP + N-acetly-D-glucosaminyl-[Mamld1 protein]
-
-
-
?
UDP-GlcNAc + MYPT1
MYPT1-GlcNAc + UDP
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + Nav1 protein
UDP + N-acetly-D-glucosaminyl-[Nav1 protein]
-
-
-
?
UDP-GlcNAc + Ncoa1 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa1 protein]
-
-
-
?
UDP-GlcNAc + Ncoa2 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa2 protein]
-
-
-
?
UDP-GlcNAc + Ncoa5 protein
UDP + N-acetly-D-glucosaminyl-[Ncoa5 protein]
-
-
-
?
UDP-GlcNAc + NFATc1
O-GlcNAc-NFATc1 + UDP
-
-
-
-
?
UDP-GlcNAc + Nfia protein
UDP + N-acetly-D-glucosaminyl-[Nfia protein]
-
-
-
?
UDP-GlcNAc + Notch epidermal growth factor 20 repeat
UDP + N-acetyl-D-gluosaminyl-[Notch epidermal growth factor 20 repeat]
-
-
-
?
UDP-GlcNAc + Nr3c1 protein
UDP + N-acetly-D-glucosaminyl-[Nr3c1 protein]
-
-
-
?
UDP-GlcNAc + Nup 62 protein
UDP + ?
-
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosaminyl-[Nup62 protein]
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosmainyl-[Nup62 protein]
UDP-GlcNAc + O-GlcNAcase
O-GlcNAcase-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + p62 protein
UDP + N-acetyl-D-glucosaminyl-[p62 protein]
-
-
-
?
UDP-GlcNAc + PGC-1alpha
UDP + N-acetyl-D-gluosaminyl-[PGC-1alpha]
-
-
-
?
UDP-GlcNAc + PGGSTPVS(PO3)-SANMM
? + UDP
-
-
-
-
?
UDP-GlcNAc + PGGSTPVSSANMM
UDP + PGGSTPV-(GlcNAc)SSANMM
-
PGGSTPVSSANMM is the best acceptor
-
-
?
UDP-GlcNAc + Plec protein
UDP + N-acetly-D-glucosaminyl-[Plec protein]
-
-
-
?
UDP-GlcNAc + Psd3 protein
UDP + N-acetly-D-glucosaminyl-[Psd3 protein]
-
-
-
?
UDP-GlcNAc + Rapgef2 protein
UDP + N-acetly-D-glucosaminyl-[Rapgef2 protein]
-
-
-
?
UDP-GlcNAc + Rasgrf2 protein
UDP + N-acetly-D-glucosaminyl-[Rasgrf2 protein]
-
-
-
?
UDP-GlcNAc + SAP130
SAP130-GlcNAc + UDP
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + Sirt2 protein
UDP + N-acetly-D-glucosaminyl-[Sirt2 protein]
-
-
-
?
UDP-GlcNAc + Ss18l1 protein
UDP + N-acetly-D-glucosaminyl-[Ss1811 protein]
-
-
-
?
UDP-GlcNAc + Stat3 protein
UDP + N-acetly-D-glucosaminyl-[Stat3 protein]
-
-
-
?
UDP-GlcNAc + Tab1 protein
UDP + N-acetly-D-glucosaminyl-[Tab1 protein]
-
-
-
?
UDP-GlcNAc + TAB1 protein
UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
UDP-GlcNAc + tau protein
GlcNAc-tau protein + UDP
-
-
-
-
?
UDP-GlcNAc + Tbr1 protein
UDP + N-acetly-D-glucosaminyl-[Tbr1 protein]
-
-
-
?
UDP-GlcNAc + Tle4 protein
UDP + N-acetly-D-glucosaminyl-[Tle4 protein]
-
-
-
?
UDP-GlcNAc + Tnik protein
UDP + N-acetly-D-glucosaminyl-[Tnik protein]
-
-
-
?
UDP-GlcNAc + TRAK1 protein
UDP + N-acetyl-D-glucosaminyl-[TRAK1 protein]
UDP-GlcNAc + transcription factor FoxM1
O-GlcNAc-transcription factor FoxM1 + UDP
-
-
-
-
?
UDP-GlcNAc + transcription factor NFAT
UDP + N-acetyl-D-glucosaminyl-[transcription factor NFAT]
-
-
-
-
?
UDP-GlcNAc + transcription factor NFkappaB
UDP + N-acetyl-D-glucosaminyl-[transcription factor NFkappaB]
-
-
-
-
?
UDP-GlcNAc + YPGGSTPVSSANMM
UDP + YPGGSTPVS-3-O-(N-acetyl-D-glucosaminyl)-SANMM
-
-
-
?
UDP-GlcNAc + YSDSPSTST
UDP + ?
UDP-GlcNAc + YSDSPSTST
UDP + GlcNAc-YSDSPSTST
-
-
-
?
UDP-GlcNAc + YSDSPSTST
YSDSP-(GlcNAc)STST + UDP
-
coupled enzyme assay of C-654
-
-
?
UDP-GlcNAc + YSPTSPSYSPTSPS
UDP + Y-(GlcNAc)SPT-(GlcNAc)SPSYSPT-(GlcNAc)SPS
-
YSPTSPSYSPTSPS is a poor substrate
-
-
?
UDP-GlcNAc + Znf532 protein
UDP + N-acetly-D-glucosaminyl-[Znf532 protein]
-
-
-
?
UDP-GlcNAc + [YSPTSPSYSPTSPS]5
UDP + [Y-(GlcNAc)SP-(GlcNAc)TSPSYSP-(GlcNAc)TSPS]5
-
-
-
-
?
UDP-GlcNAc DELTA491 protein
UDP + N-acetyl-D-glucosaminyl-[DELTA491 protein]
-
N-terminal truncation of OIP106, able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + FITC-YAVVPVSK peptide
UDP + ?
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [Nup62 protein]-L-serine
UDP + [Nup62 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
-
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
UDP-N-acetyl-D-glucosamine + KKKYPGGSTPVSSANMM
UDP + ?
-
-
-
?
UDP-N-acetyl-D-glucosamine + YPGGSTPVSSANMM
UDP + YPGGSTPVS-3-O-(N-acetyl-D-glucosaminyl)-SANMM
-
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
?
UDP-N-acetyl-5-deoxy-5-thio-alpha-D-glucosamine is a very poor (3200times slower) donor substrate compared to UDP-N-acetyl-D-glucosamine
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine
the enzyme transfers N-acetylglucosamine from the sugar donor UDP-GlcNAc onto specific serine or threonine residues of nucleocytoplasmic proteins with inversion of configuration at the anomeric center
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-serine
UDP + [protein]-O3-(N-acetyl-D-glucosaminyl)-L-serine
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + [protein]-L-threonine
UDP + [protein]-O3-(N-acetyl-D-glucosaminyl)-L-threonine
-
-
-
-
?
YSDSPSTST + UDP-GlcNAc
UDP + ?
-
-
-
-
?
additional information
?
-
adhesin PsrP + UDP-GlcNAc
? + UDP
-
the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity
-
?
adhesin PsrP + UDP-GlcNAc
? + UDP
-
the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the first serine-rich repeat of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Formation of the GtfA-GtfB complex is crucial for the O-GlcNAcylation activity
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
?
capsid protein of Plum pox virus + UDP-N-acetyl-D-glucosamine
UDP + N-acetyl-D-glucosaminyl-[capsid protein of Plum pox virus]
-
-
-
-
?
UDP-GlcNAc + CKII peptide
UDP + N-acetyl-D-glucosaminyl-[CKII peptide]
-
-
-
?
UDP-GlcNAc + CKII peptide
UDP + N-acetyl-D-glucosaminyl-[CKII peptide]
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase
glutathione S-transferase-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase
glutathione S-transferase-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-CARM1
glutathione S-transferase-CARM1-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-CARM1
glutathione S-transferase-CARM1-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-MYPT1
glutathione S-transferase-MYPT1-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + glutathione S-transferase-MYPT1
glutathione S-transferase-MYPT1-GlcNAc + UDP
-
-
-
-
?
UDP-GlcNAc + H2O
UDP + GlcNAc
-
-
-
?
UDP-GlcNAc + H2O
UDP + GlcNAc
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosmainyl-[Nup62 protein]
-
-
-
-
?
UDP-GlcNAc + Nup62 protein
UDP + N-acetyl-D-glucosmainyl-[Nup62 protein]
-
control substrate
-
-
?
UDP-GlcNAc + TAB1 protein
UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
-
-
-
?
UDP-GlcNAc + TAB1 protein
UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
-
-
-
-
?
UDP-GlcNAc + TAB1 protein
UDP + N-acetyl-D-glucosaminyl-[TAB1 protein]
-
-
-
-
?
UDP-GlcNAc + TRAK1 protein
UDP + N-acetyl-D-glucosaminyl-[TRAK1 protein]
-
putative OGT binding partner interact with OGT when co-expressed in yeast (yeast two-hybrid screen)
-
-
?
UDP-GlcNAc + TRAK1 protein
UDP + N-acetyl-D-glucosaminyl-[TRAK1 protein]
-
-
-
-
?
UDP-GlcNAc + YSDSPSTST
UDP + ?
-
-
-
-
?
UDP-GlcNAc + YSDSPSTST
UDP + ?
-
highly specific synthetic peptide assay, affinity of the enzyme for UDP-GlcNAc is high and for the peptide substrate, YSDSPSTST, is only moderate
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
octamer-binding protein 4 (Oct4) is one of the key transcription factors required for pluripotency of embryonic stem cell and more recently, the generation of induced pluripotent stem cells. The action of Oct4 is modulated by the addition of several post-translational modifications, including O-GlcNAc. Human Oct4 activity is regulated by O-linked N-acetylglucosamine transferase by a mechanism that is distinct from mouse Oct4
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [octamer-binding protein 4]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
octamer-binding protein 4 (Oct4) is one of the key transcription factors required for pluripotency of embryonic stem cell and more recently, the generation of induced pluripotent stem cells. The action of Oct4 is modulated by the addition of several post-translational modifications, including O-GlcNAc. The action of Oct4 is modulated by the addition of several post-translational modifications, including O-GlcNAc. Human Oct4 activity is regulated by O-linked N-acetylglucosamine transferase by a mechanism that is distinct from mouse Oct4
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
essential enzyme that catalyzes the covalent bonding of N-acetylglucosamine to the hydroxyl group of a serine or threonine in the target protein. It plays an important role in many important cellular physiological catalytic reactions
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Lys634, Asn838, Gln839, Lys842, His901, and Asp925 play an important role in stabilizing UDP at the active site of the enzyme via hydrogen bonds and pi-pi interactions. The binding free energy of the UDP-enzyme complex is mainly constituted by electrostatic interactions and side chain effects. The uridine diphosphate release mechanism is studied
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UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
the enzyme recognizes the majority of its substrates by binding them to the asparagine ladder within the lumen of superhelical tetratricopeptide repeat (TPR) domain of the enzyme
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UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
essential enzyme that catalyzes the covalent bonding of N-acetylglucosamine to the hydroxyl group of a serine or threonine in the target protein. It plays an important role in many important cellular physiological catalytic reactions
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UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
Lys634, Asn838, Gln839, Lys842, His901, and Asp925 play an important role in stabilizing UDP at the active site of the enzyme via hydrogen bonds and pi-pi interactions. The binding free energy of the UDP-enzyme complex is mainly constituted by electrostatic interactions and side chain effects. The uridine diphosphate release mechanism is studied
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UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine
UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine
the enzyme recognizes the majority of its substrates by binding them to the asparagine ladder within the lumen of superhelical tetratricopeptide repeat (TPR) domain of the enzyme
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catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
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catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to Ser/Thr residues of proteins
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enzyme adds a single GlcNAc to hydroxyl groups of serine and threonine residues. In the plant Arabidopsis, OGT serves to attenuate the gibberillin pathway
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enzyme modifies the Plum pox virus capsid protein
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enzyme modifies the Plum pox virus capsid protein
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enzyme modifies the Plum pox virus capsid protein
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participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
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participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
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participates in plum pox virus infection, plants are inoculated with PPV-NK-GFP and observed at different times postinoculation under a fluorescence microscope
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p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc are no substrates. The cloned enzyme cleaves GlcNAc, but not GalNAc, from glycopeptides
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heat shock protein 90 (Hsp90) is involved in the regulation of O-linked beta-N-acetylglucosamine transferase. Inhibition of Hsp90 by radicicol or 17-N-allylamino-17-demethoxygeldanamycin destabilizes the enzyme and dramatically reduces its half-life in primary cultures of endothelial cells
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catalyzes the transfer of O-linked GlcNAc to serine or threonine residues of a variety of substrate proteins, including nuclear pore proteins, transcription factors, and proteins implicated in diabetes and neurodegenerative disorders
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catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration
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enzyme transfers N-acetylglucosamine from UDP-GlcNAc to selected serine and threonine residues
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regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1
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regulation of O-GlcN acylation over a broad range of glucose concentrations, significant induction of O-GlcNAc modification of a limited number of proteins under conditions of glucose deprivation
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transfer of O-linked GlcNAc to serine/threonine residues of a variety of substrate proteins, including nuclear pore proteins, transcription factors, and proteins implicated in diabetes and neurodegenerative disorders
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enzyme adds a single GlcNAc to hydroxyl groups of serine and threonine residues. Substrates are many proteins, e.g. transcription factors, kinases, cytoskeletal proteins, and nuclear pore proteins
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enzyme catalyzes O-GlcNAc addition
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enzyme catalyzes the addition of O-GlcNAc moieties to nuclear and cytoplasmic proteins at serine and threonine residues, regulates some aspects of mitotic chromatin dynamics. OGT protein amounts decrease during M phase
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enzyme is a key molecule for the timely progression of the cell cycle. Microinject recombinant proteins into oocytes to detail the relationship between cell cycle and O-GlcNAc
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enzyme modifies nuclear pore proteins and transcription factors
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enzyme transfers GlcNAc onto substrate proteins using UDP-GlcNAc as the sugar donor
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enzyme does not modify peptide YSDSGSTST, cAMP-dependent protein kinase A, casein kinase I, mitogen activated protein kinase (extracellular signal-regulated kinase 2), or calmodulin-dependent protein kinase II
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UDP-1-deoxy-1-thio-N-acetyl-alpha-D-glucosamine is no substrate
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heat shock protein 90 (Hsp90) is involved in the regulation of O-linked beta-N-acetylglucosamine transferase. Inhibition of Hsp90 by radicicol or 17-N-allylamino-17-demethoxygeldanamycin destabilizes the enzyme and dramatically reduces its half-life in primary cultures of endothelial cells
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heat shock protein 90 (Hsp90) is involved in the regulation of O-linked beta-N-acetylglucosamine transferase. Inhibition of Hsp90 by radicicol or 17-N-allylamino-17-demethoxygeldanamycin destabilizes the enzyme and dramatically reduces its half-life in primary cultures of endothelial cells
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no activity with Tau protein
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no activity with Tau protein
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the enzyme is not able to transfer UDP-glucose or UDP-2-dehydro-alpha-D-glucose to peptide and protein substrates
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a combination of size and conformational restriction defines sequence specificity in the -3 to +2 subsites of O-GlcNAc modification. Although the N-terminal tetratricopeptide repeats of OGT may have roles in substrate recognition, the sequence restriction imposed by the peptide-binding site makes a substantial contribution to O-GlcNAc site specificity
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modification occurs predominantly in a random coil region, with signature sequence, PPVS/TSATT, around the modification site (underlined, position 0). A substrate (peptide or protein) with Pro, Ala at position -2, and/or Val, Ala, Thr, Ser at position -1, and/or Ala, Ser, Pro, Thr, Gly at position +2 would have more chances for O-GlcNAcylation
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the substitution of the N-acyl group, deoxy modification of C6/C4-OH or epimerization of C4-OH of the GlcNAc in UDP-GlcNAc decrease its affinity to isoform short OGT. The backbone carbonyl oxygen of Leu653 and the hydroxyl group of Thr560 in sOGT contribute to the recognition of the sugar moiety via hydrogen bonds
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the substitution of the N-acyl group, deoxy modification of C6/C4-OH or epimerization of C4-OH of the GlcNAc in UDP-GlcNAc decrease its affinity to isoform short OGT. The backbone carbonyl oxygen of Leu653 and the hydroxyl group of Thr560 in sOGT contribute to the recognition of the sugar moiety via hydrogen bonds
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aspartate residues far from the active site drive O-GlcNAc transferase substrate selection
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the enzyme is quite promiscuous for its donor sugar substrates. It can endogenously modify proteins with both N-acetyl-glucosamine and glucose
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enzyme catalyzes the addition of O-linked beta-N-acetylglucosamine (O-GlcNAc) onto serine and threonine residues in response to stimuli or stress analogous to phosphorylation by Ser/Thr-kinases
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no activity with UDP-GalNAc, UDP-D-glucose, UDP-D-galactose, and UDP-D-xylose
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no activity with UDP-GalNAc, UDP-D-glucose, UDP-D-galactose, and UDP-D-xylose
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participates in plum pox virus infection, plants are inoculated with plum pox virus-NK-GFP and observed at different times postinoculation under a fluorescence microscope
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catalyses the O-linked attachment of single GlcNAc moieties to serine and threonine residues on many cytosolic or nuclear proteins
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catalyzes the attachment of GlcNAc monosaccharides to the hydroxyl group of serine or threonine residues of intracellular proteins and may play an important role in the hexosamine pathway
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enzyme catalyzes the abundant and dynamic posttranslational modification of nuclear and cytosolic proteins by beta-O-linked N-acetylglucosamine (O-GlcNAc)
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O-GlcNAc transferase, the enzyme that adds O-GlcNAc to proteins, exists in stable and active complexes with the serine/threonine phosphatases PP1beta and PP1gamma, enzymes that remove phosphate from proteins
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p110 subunit of the enzyme forms both homo- and heterotrimers that appear to have different binding affinities for UDP-GlcNAc
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DELTA859 is not able to bind and pull down OGT, indicates that the potential OGT-binding domain localized to within residues 639-859 in the C-terminus of OIP106
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OGT-interacting proteins interact strongly with the tetratricopeptide repeat (TPR) domain of OGT, they are modified by O-GlcNAc and are excellent substrates of OGT
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enzyme adds a single GlcNAc to hydroxyl groups of serine and threonine residues
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enzyme is able to hydrolyze UDP-GlcNAc
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enzyme is able to hydrolyze UDP-GlcNAc
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enzyme catalyzes O-GlcNAc addition
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enzyme transfers GlcNAc onto substrate proteins using UDP-GlcNAc as the sugar donor
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UDP-1-deoxy-1-thio-N-acetyl-alpha-D-glucosamine is no substrate
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