Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | increases the activity of wild type OGT | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C836S | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type | Homo sapiens |
C839S | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
D407A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
D422A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I, produces a 50-100% increase in activity | Homo sapiens |
D438A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
D488A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
D505A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
D549A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
D554A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
E482A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
E556A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
E568A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
F439A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
F460A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
F721A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type | Homo sapiens |
F752A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 110% activity compared to wild type | Homo sapiens |
F776A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, 10% activity compared to wild type | Homo sapiens |
G402S | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
G453S | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
G472A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
G538S | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
L796A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
additional information | following mutations are not included for enzymatic analysis, because not sufficient amounts of protein could be produced: Y387A, F439A, D505A, W536A, Y539A, D549A, D554A, and E556A | Homo sapiens |
additional information | site-directed mutagenesis to target potentially important amino acid residues within the two conserved catalytic domains of OGT (CD I and CD II), followed by an in vitro glycosylation assay to evaluate N-acetylglucosaminyltransferase activity after bacterial expression | Homo sapiens |
W536A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
W735A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
W748A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
W812A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
W878A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD II, less than 1% activity compared to wild type | Homo sapiens |
Y387A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I, mutation is not included for enzymatic analysis, because not sufficient amounts of protein could be produced | Homo sapiens |
Y434A | site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
Y539A | significant inhibitory effect on OGT enzyme activity, site-directed mutagenesis to target potentially important amino acid residues within the conserved catalytic domain CD I | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alloxan | - |
Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
UDP-GlcNAc | F752A mutant | Homo sapiens | |
0.29 | - |
UDP-GlcNAc | D422A mutant | Homo sapiens | |
0.44 | - |
UDP-GlcNAc | F776A mutant | Homo sapiens | |
0.8 | - |
UDP-GlcNAc | mOGT wild type | Homo sapiens | |
1.9 | - |
UDP-GlcNAc | Y434A mutant, approximately double the Km value of wild type | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalyzes the transfer of O-linked GlcNAc to serine/threonine residues of a variety of target proteins, many of which have been implicated in such diseases as diabetes and neurodegeneration | Homo sapiens | ? | - |
? | |
UDP-GlcNAc + Nup62 protein | - |
Homo sapiens | UDP + N-acetyl-D-glucosmainyl-[Nup62 protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mOGT | mitochondrial OGT | Homo sapiens |
O-linked N-acetylglucosaminyltransferase | - |
Homo sapiens |
OGT | - |
Homo sapiens |