Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants of OGT variant A-G. Deletions in the highly conserved C-terminus result in a complete loss of activity. The N-terminal tetratricopeptide repeat domain is required for optimal recognition of substrates. Removal of the first three tetratricopeptide repeats greatly reduces the O-GlcNAc addition to macromolecular substrates | Homo sapiens |
additional information | deletion of the first 3 tetratricopeptide repeats in clone B results in a significant loss of activity (58%) for Nup 62 | Homo sapiens |
additional information | removal of the first 6 tetratricopeptide repeats in clone C results in an almost complete loss of activity toward Nup 62 as a substrate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | completely inhibiting | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
UDP-GlcNAc | - |
Homo sapiens | |
0.0012 | - |
Nup 62 protein | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
- |
Homo sapiens |
114000 | - |
fusion protein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme modifies nuclear pore proteins and transcription factors | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
clone Lv4F | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein kinase II peptide + UDP-GlcNAc | much poorer substrate than Nup 62 | Homo sapiens | UDP + ? | - |
? | |
GSK-3beta + UDP-GlcNAc | rabbit skeletal muscle glycogen synthase kinase (GSK) -3beta | Homo sapiens | UDP + ? | - |
? | |
additional information | enzyme modifies nuclear pore proteins and transcription factors | Homo sapiens | ? | - |
? | |
additional information | enzyme does not modify peptide YSDSGSTST, cAMP-dependent protein kinase A, casein kinase I, mitogen activated protein kinase (extracellular signal-regulated kinase 2), or calmodulin-dependent protein kinase II | Homo sapiens | ? | - |
? | |
UDP-GlcNAc + Nup 62 protein | - |
Homo sapiens | UDP + ? | - |
? | |
YSDSPSTST + UDP-GlcNAc | - |
Homo sapiens | UDP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
human OGT | - |
Homo sapiens |
O-linked GlcNAc transferase | - |
Homo sapiens |
OGT | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |