2.4.1.255: protein O-GlcNAc transferase This is an abbreviated version! For detailed information about protein O-GlcNAc transferase, go to the full flat file .
Word Map on EC 2.4.1.255
Reaction
UDP-N-acetyl-alpha-D-glucosamine +
[protein]-L-serine =
UDP +
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Synonyms beta-N-acetylglucosaminidase, EGF domain-specific O-GlcNAc transferase, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, EOGT1, epidermal growth factor domain-specific O-GlcNAc transferase, glycosyltransferase OGT, Gtf1, GtfA, hOGT, HsOGT, human OGT, mammalian OGT, mOGT, More, N-acetylglucosamine transferase, N-acetylglucosamine-peptide N-acetylglucosaminyltransferase, N-acetylglucosaminyltransferase, ncOGT, nuclear cytoplasmic OGT, nucleocytoplasmic glycosyltransferase, nucleocytoplasmic OGT, O-GlcNAc protein, O-GlcNAc transferase, O-GlcNAc transferase OGT, O-GlcNAc-transferase, O-GlcNAcase, O-glycosylated protein p135, O-linked beta-N-acetylglucosamine transferase, O-linked beta-N-acetylglucosaminyltransferase, O-linked GlcNAc transferase, O-linked N-acetylglucosamine transferase, O-linked N-acetylglucosaminyltransferase, O-N-acetylglucosamine (O-GlcNAc) transferase, OGT, OGT p110, OGT protein, ogta, OGTase, OGTase protein, ogtb, p135, Protein SECRET AGENT, SEC, SECRET AGENT, sOGT, spindly, SPY, super sex combs, Sxc, Synpcc7942_0051, transferase OGT, UDP-GlcNAc: polypeptide O-N-acetylglucosaminyltransferase, UDP-GlcNAc:polypeptidyl transferase, UDP-N-acetylglucosaminyl transferase, uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylaminyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase, XcOGT, XcOGT protein
ECTree
Activating Compound
Activating Compound on EC 2.4.1.255 - protein O-GlcNAc transferase
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CoCl2
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stress treatment induces higher levels of OGT protein expression
D-glucose
immunoblot analysis of OGTase expression in RASM cells. The amount of the 78 kDa subunit is significantly increased in the high glucose, but no significant increase in the amount of the 110 kDa subunit is observed. OGTase expression and activity increased in the RASM cells cultured in the 20 mM glucose
dithiothreitol
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increases the activity of wild type OGT
ethanol
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stress treatment induces higher levels of OGT protein expression
H2O2
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stress treatment induces higher levels of OGT protein expression
hyperglycemia
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increases O-GlcNAc levels in pancreatic beta cells, which appears to interfere with beta-cell function
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Insulin
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tyrosine phosphorylation of OGT increases significantly after a 10 min insulin treatment
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NaCl
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stress treatment induces higher levels of OGT protein expression
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenylcarbamate
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inhibitor of O-GlcNAcase, increase of the level of O-GlcNAc modifications
sodium arsenite
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stress treatment induces higher levels of OGT protein expression
UDP-N-acetyl-D-glucosamine
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UVB light
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stress treatment induces higher levels of OGT protein expression
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vanadate
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tyrosine phosphatase inhibitor sodium vanadate, treatment increases OGT activity. When insulin is added to cells preincubated with sodium vanadate, there is a further increase in OGT activity versus inhibitor alone
Ataxin-10
Atx-10 increases OGT activity 2fold
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Ataxin-10
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encoded by the SCA10 (spinocerebellar ataxia type 10) gene, interacts with O-GlcNAc transferase OGT in pancreatic beta cells
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Ataxin-10
Atx-10 increases OGT activity 2fold
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additional information
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beginning 12 h after treatment, glucose deprived human hepatocellular carcinoma (HepG2) cells demonstrate a 7.8fold increase in total O-GlcNAc modification compared with cells cultured in normal glucose (5 mM)
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additional information
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GPX1 activity is increased about 0.5fold by OGT
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additional information
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insulin signaling stimulates tyrosine phosphorylation of OGT, increases its catalytic activity, affects OGT localization, and results in the O-GlcNAc modification of STAT3, a key kinase and transcription factor in 3T3-L1 adipocytes
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additional information
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nearly all of the peptides became better acceptors as the concentration of UDP-GlcNAc increased with the exception of the YSPTSPSYSPTSPS
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