1.14.20.6: flavonol synthase
This is an abbreviated version!
For detailed information about flavonol synthase, go to the full flat file.
Word Map on EC 1.14.20.6
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1.14.20.6
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flavonols
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flavanone
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chalcone
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dihydroflavonols
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kaempferol
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anthocyanidins
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4-reductase
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dihydrokaempferol
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3\'-hydroxylase
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leucoanthocyanidin
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r2r3-myb
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dihydroquercetin
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3beta-hydroxylase
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fagopyrum
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tataricum
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2-odds
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2s-naringenin
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dihydroflavonol-4-reductase
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unshius
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analysis
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synthesis
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tartary
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flavonol-specific
- 1.14.20.6
- flavonols
- flavanone
- chalcone
- dihydroflavonols
- kaempferol
- anthocyanidins
-
4-reductase
- dihydrokaempferol
-
3\'-hydroxylase
-
leucoanthocyanidin
-
r2r3-myb
- dihydroquercetin
-
3beta-hydroxylase
- fagopyrum
- tataricum
- 2-odds
- 2s-naringenin
- dihydroflavonol-4-reductase
- unshius
- analysis
- synthesis
-
tartary
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flavonol-specific
Reaction
Synonyms
AaFLS1, BnFLS, Cs-COR054, Cs-COR113, EC 1.14.11.23, FKS2, flavonoid 2-oxoglutarate-dependent dioxygenase, flavonol synthase, FLS, FLS1, FLS2, fls3, McFLS, NtFLS1, PhFLS, PpFLS, RrFLS1, SbFLS, VcFLS
ECTree
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Engineering
Engineering on EC 1.14.20.6 - flavonol synthase
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A120M
the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 162% of the catalytic activity relative to wild-type enzyme
F146I
the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 86% of the catalytic activity relative to wild-type enzyme
L311F
the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 355% of the catalytic activity relative to wild-type enzyme
Y240P
the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 104% of the catalytic activity relative to wild-type enzyme
Y240P/A120M
the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 175% of the catalytic activity relative to wild-type enzyme
H132F
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site-directed mutagenesis, the mutant shows similar kinetics with dihydrokaempferol compared to the wild-type enzyme
additional information
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wild-type, fls1-2 single mutant and ldox/fls1-2 double mutant, lacking acitve form of flavonol synthase and leucoanthocyanidin dioxygenase, EC 1.14.11.19
additional information
a single base deletion in the flavonol synthase gene is responsible for the magenta flower mutant Harosoy due to a truncated polypeptide that lacks the dioxygenase domains A and B
additional information
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a single base deletion in the flavonol synthase gene is responsible for the magenta flower mutant Harosoy due to a truncated polypeptide that lacks the dioxygenase domains A and B
additional information
silencing of FLS gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview
additional information
silencing of FLS gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview
additional information
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silencing of FLS gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview
additional information
silencing of FLS gene using an ihpRNA construct from gene sequences of FLS and FLS1 and transferred to Agrobacterium tumefaciens strain LBA4404 through tri-parental mating, Agrobacterium tumefaciens-mediated transfection, phenotype, overview
additional information
silencing of FLS gene using an ihpRNA construct from gene sequences of FLS and FLS1 and transferred to Agrobacterium tumefaciens strain LBA4404 through tri-parental mating, Agrobacterium tumefaciens-mediated transfection, phenotype, overview
additional information
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silencing of FLS gene using an ihpRNA construct from gene sequences of FLS and FLS1 and transferred to Agrobacterium tumefaciens strain LBA4404 through tri-parental mating, Agrobacterium tumefaciens-mediated transfection, phenotype, overview
additional information
silencing of FLS1 gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview
additional information
silencing of FLS1 gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview
additional information
-
silencing of FLS1 gene by RNAi, in lines G12, A2, B1 and E13, the quercetin content is increased by 351%, 49%, 157% and 258%, the silenced lines show arrest in pollen tube germination, and the pollen tubes grows only to about nine-tenths of the way down the style, and tube tips are swollen, contents of anthocyanins, epigallocatechin, and epicatechin are altered and content in indole acetic acid is reduced compared to the wild-type, overview