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1.14.20.6: flavonol synthase

This is an abbreviated version!
For detailed information about flavonol synthase, go to the full flat file.

Word Map on EC 1.14.20.6

Reaction

a dihydroflavonol
+
2-oxoglutarate
+
O2
=
a flavonol
+
succinate
+
CO2
+
H2O

Synonyms

AaFLS1, BnFLS, Cs-COR054, Cs-COR113, EC 1.14.11.23, FKS2, flavonoid 2-oxoglutarate-dependent dioxygenase, flavonol synthase, FLS, FLS1, FLS2, fls3, McFLS, NtFLS1, PhFLS, PpFLS, RrFLS1, SbFLS, VcFLS

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated
                1.14.20.6 flavonol synthase

Cloned

Cloned on EC 1.14.20.6 - flavonol synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21Star
-
expressed with a glutathione-S-transferase tagging in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli as glutathione S-transferase fusion protein
expression in Escherichia coli as thioredoxin fusion proteins
expression in Escherichia coli JM109
expression in Escherichia coli Transetta (DE3)
expression in Escherichia coli, His-tag
expression in Saccharomyces cereviseae strain INV Sc1 and in Escherichia coli BL21-A1
-
expression in yeast
flavonol synthase is introduced and overexpressed into Arabidopsis plants. Overexpression of flavonol synthase leads to a higher accumulation of flavonols and results in lower anthocyanin levels in these transgenic plants. overexpression confers increased antioxidative capability to transgenic oilseed plants
-
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
-
gene AcFLS, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged FLS protein in Escherichia coli strain BL21-CodonPlus-RIL
gene FtFLS1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene FtFLS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative expression analysis
gene FtFLS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
gene FtFLS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative expression analysis
gene GbFLS, DNA and amino acid sequence determination and analysis, promoter region sequence analysis and putative cis-acting regulatory elements, overview, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ZmFLS1, ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators in vivo, recombinant expression as His-tagged protein in Escherichia coli
-
overexpression of AaFLS1 in Nicotiana tabacum
-
overexpression of the heterologous flavonol synthase genes in tobacco substantially inhibits expression of the Nicotiana tabacum gene in the flowers and upregulates expression of the endogenous dihydroflavonol-4-reductase gene. Heterologous expression of the flavonol synthase genes within tobacco host plants demonstrates conservation of function, with the transgenes promoting flavonol biosynthesis and inhibiting anthocyanin accumulation, so resulting in white flowers