1.14.18.11: plant 4alpha-monomethylsterol monooxygenase
This is an abbreviated version!
For detailed information about plant 4alpha-monomethylsterol monooxygenase, go to the full flat file.
Reaction
Synonyms
4alpha-methyl oxidase, 4alpha-methyl-DELTA7-sterol-4alpha-methyl-oxidase, 4alpha-methylsterol C4 oxidase, 4alpha-methylsterol-4alpha-methyl-oxidase, 4alpha-MSO, methylsterol monooxygenase 2, SMO2, SMO2-1, SMO2-2, sterol-4alpha-methyl oxidase 2
ECTree
1.14.18.11 plant 4alpha-monomethylsterol monooxygenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.14.18.11 - plant 4alpha-monomethylsterol monooxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methyl-9,19-cycloergosta-7,24(28)-dien-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxy-9,19-cycloergosta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
ir
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-4-methylcholest-7-en-3-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
(3alpha,4alpha,5alpha,10alpha,13alpha,20S)-3-hydroxycholest-7-ene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
ir
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
(3beta,4alpha,5alpha,24Z)-3-hydroxystigmasta-7,24(28)-diene-4-carboxylic acid + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
ir
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta-hydroxyergosta-7,24(241)-dien-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
ir
24-methylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta-hydroxyergosta-7,24(241)-diene-4alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
?
24-methylidenelophenol + NAD(P)H + H+
?
-
59% activity compared to 24-ethylidenelophenol
-
-
?
24zeta(241)-dihydrocycloeucalenol + NAD(P)H + H+
?
-
10% activity compared to 24-ethylidenelophenol
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
cycloeucalenol + NAD(P)H + H+
?
-
10% activity compared to 24-ethylidenelophenol
-
-
?
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
?
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
?
24-ethylidenelophenol + 6 ferrocytochrome b5 + 3 O2 + 6 H+
3beta,4alpha,5alpha-3-hydroxystigmasta-7,24(28)-diene-4-carboxylate + 6 ferricytochrome b5 + 4 H2O
-
-
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
step 3 of 3 of the overall reaction
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
step 3 of 3 of the overall reaction
-
-
?
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carboxylate + a ferricytochrome + H2O
step 3 of 3 of the overall reaction
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
step 1 of 3 of the overall reaction
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
step 1 of 3 of the overall reaction
-
-
?
4,4-dimethyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferricytochrome + H2O
step 1 of 3 of the overall reaction
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
step 2 of 3 of the overall reaction
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
step 2 of 3 of the overall reaction
-
-
?
4beta-hydroxymethyl-4alpha-methyl-5alpha-cholest-7-en-3beta-ol + a ferrocytochrome + O2
3beta-hydroxy-4beta-methyl-5alpha-cholest-7-ene-4alpha-carbaldehyde + a ferricytochrome + H2O
step 2 of 3 of the overall reaction
-
-
?
additional information
?
-
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
-
-
?
additional information
?
-
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
-
-
?
additional information
?
-
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO, sterol spectrum in wild-type and transgenic gene-silenced plants, accumulation of 4alpha-methyl-DELTA7-sterols, overview
-
-
?
additional information
?
-
conversion of cycloartenol into functional phytosterol requires the removal of 2 methyl groups at C4 by the enzymic complex including the sterol 4alpha-methyl oxidase SMO
-
-
?
additional information
?
-
-
no activity with cyclobranol, cycloartenol, 24-methylenelanosterol, lanosterol, 24(25)-dihydrolanosterol, parkeol, 4,4-dimethyl-7,22-ergostadienol, beta-amyrine, 4alpha,14alpha,dimethyl-5alpha-cholesta-7-en-3beta-ol, obtusifoliol, 24zeta(241)-dihydroobtusifoliol, 29-norlanosterol, 24(25)-dihydro-29-norlanosterol, 4alpha-dimethyl-5alpha-cholestanol, 4alpha,14alpha,24zeta-trimethyl-5alpha-cholesta-9(11)-en-3beta-ol, and 4beta-24zeta(241)-dihydrocycloeucalenol
-
-
?
