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1.14.18.11: plant 4alpha-monomethylsterol monooxygenase

This is an abbreviated version!
For detailed information about plant 4alpha-monomethylsterol monooxygenase, go to the full flat file.

Reaction

24-methylidenelophenol
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
4alpha-(hydroxymethyl)ergosta-7,24(241)-dien-3beta-ol
+ 2 ferricytochrome b5 +
H2O

Synonyms

4alpha-methyl oxidase, 4alpha-methyl-DELTA7-sterol-4alpha-methyl-oxidase, 4alpha-methylsterol C4 oxidase, 4alpha-methylsterol-4alpha-methyl-oxidase, 4alpha-MSO, methylsterol monooxygenase 2, SMO2, SMO2-1, SMO2-2, sterol-4alpha-methyl oxidase 2

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.11 plant 4alpha-monomethylsterol monooxygenase

Cloned

Cloned on EC 1.14.18.11 - plant 4alpha-monomethylsterol monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression in enzyme-lacking Saccharomyces cerevisiae strain erg25
DNA and amino acid sequence determination and analysis, in vitro transcription
expressed in Nicotiana benthamiana
-
expressed in Saccharomyces cerevisiae erg25 ergosterol auxotroph strain