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1.14.19.64: (S)-stylopine synthase

This is an abbreviated version!
For detailed information about (S)-stylopine synthase, go to the full flat file.

Word Map on EC 1.14.19.64

Reaction

(S)-cheilanthifoline
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
(S)-Stylopine
 +
[oxidized NADPH-hemoprotein reductase]
 + 2 H2O

Synonyms

(S)-cheilanthifoline oxidase (methylenedioxy-bridge-forming), CYP719A13, CYP719A2, CYP719A2/A3, CYP719A3, EC 1.1.3.32, EC 1.14.21.1, methylenedioxy bridge-forming enzyme, stylopine synthase, synthase, (S)-stylopine

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.64 (S)-stylopine synthase

Cloned

Cloned on EC 1.14.19.64 - (S)-stylopine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline)
CYP719A2 and CYP719A3, DNA and amino acid sequence determination and analysis, expression patterns, expression in Saccharomyces cerevisiae microsomes
-
expressed in Spodoptera frugiperda Sf9 cells
heterologously expressed in Saccharomyces cerevisiae
two full-length P450 cDNAs, CYP719A2 and CYP7193A, expression in Saccharomyces cerevisiae
-