EC Number   |
Application |
Reference |
|---|
    2.3.3.21 | biofuel production |
advantage of the growth phenotype associated with 2-keto acid deficiency to construct a hyperproducer of 1-propanol and 1-butanol by evolving citramalate synthase (CimA) from Methanococcus jannaschii |
-, 690552 |
| 2.3.3.21 | biotechnology |
fermentation of C5 and C6 sugars to ethanols and other metabolites under thermophilic conditions |
701798 |
| 2.3.3.21 | energy production |
the citratmalate pathway is enhanced for the production of 1-propanol and 1-butanol |
-, 690552 |
| 2.3.3.21 | medicine |
catalyses the first reaction of the pathway which converts pyruvate and acetyl-CoA into citramalate, thus making it an attractive target for the development of antibacterial agents |
685015 |
| 2.3.3.21 | medicine |
Leptospira interrogans is the causative agent for leptospirosis, LiCMS is an atttractive target for the development of antibacterial agents |
685015 |
| 2.3.3.21 | synthesis |
in a fedbatch process an exponential feeding strategy using expression of CimA and the citrate synthase GltA F383M variant, over 60 g/l citramalate with a yield of 0.53 g citramalate/g glucose are generated in 132 hr |
762840 |
| 2.3.3.21 | synthesis |
synthesis of citramalate at high yield by Escherichia coli overexpressing citramalate synthase. Citramalate is a chemical precursor to the industrially important methacrylic acid. Acetate is an undesirable by-product potentially formed from pyruvate and acetyl-CoA, the precursors of citramalate during aerobic growth of Escherichia coli. Gene deletions critical to reducing acetate accumulation during aerobic growth and citramalate production are ifdentified in metabolically engineered Escherichia coli strains. The key knockouts critical to minimizing acetate formation are identified as pta, ackA and poxB |
-, 748589 |
| 2.3.3.21 | synthesis |
synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli |
-, 748351 |
| 2.3.3.21 | synthesis |
the autrotrophic micro-organism may be engineered for robust butanol and propanol production from 2-ketobutyrate, which is an intermediate in the isoleucine biosynthesis pathway |
720389 |
