Literature summary for 2.3.3.21 extracted from

Feng, X.; Mouttaki, H.; Lin, L.; Huang, R.; Wu, B.; Hemme, C.L.; He, Z.; Zhang, B.; Hicks, L.M.; Xu, J.; Zhou, J.; Tang, Y.J.
Characterization of the central metabolic pathways in Thermoanaerobacter sp. strain X514 via isotopomer-assisted metabolite analysis (2009), Appl. Environ. Microbiol., 75, 5001-5008.

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Application

Application	Comment	Organism
biotechnology	fermentation of C5 and C6 sugars to ethanols and other metabolites under thermophilic conditions	Thermoanaerobacter sp. X514

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate + acetyl-CoA + H2O	Thermoanaerobacter sp. X514	-	(2R)-2-hydroxy-2-methylbutanedioate + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermoanaerobacter sp.	B0K6M2	-	-
Thermoanaerobacter sp. X514	B0K6M2	-	-

Purification (Commentary)

Purification (Comment)	Organism
cells are centrifuged, pellet sonicated, resuspended in 0.1 M N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer, pH 7.5	Thermoanaerobacter sp. X514

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.027	-	production of CoA measured at 60°C, pH 7.5	Thermoanaerobacter sp. X514

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + pyruvate + H2O	the organism exhibits an inactive pentose phosphate pathway and an alternate isoleucine biosynthesis pathway via the citramalate pathway that uses pyruvate and acetyl-CoA as precursors	Thermoanaerobacter sp.	CoA + (R)-citramalate	-	?
pyruvate + acetyl-CoA + H2O	-	Thermoanaerobacter sp. X514	(2R)-2-hydroxy-2-methylbutanedioate + CoA	-	?

Synonyms

Synonyms	Comment	Organism
CimA	-	Thermoanaerobacter sp.
cimA protein	-	Thermoanaerobacter sp. X514
citramalate synthase	-	Thermoanaerobacter sp.
citramalate synthase	-	Thermoanaerobacter sp. X514

General Information

General Information	Comment	Organism
metabolism	biosynthesis of isoleucine from the enzyme product citramalate ((2R)-2-hydroxy-2-methylbutanedioate)	Thermoanaerobacter sp. X514
physiological function	the organism exhibits an inactive pentose phosphate pathway and an alternate isoleucine biosynthesis pathway via the citramalate pathway that uses pyruvate and acetyl-CoA as precursors	Thermoanaerobacter sp.

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Release 2023.1 (January 2023)