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Literature summary for 2.3.3.21 extracted from

  • Wu, X.; Tovilla-Coutino, D.B.; Eiteman, M.A.
    Engineered citrate synthase improves citramalic acid generation in Escherichia coli (2020), Biotechnol. Bioeng., 117, 2781-2790 .
    View publication on PubMed

Application

Application Comment Organism
synthesis in a fedbatch process an exponential feeding strategy using expression of CimA and the citrate synthase GltA F383M variant, over 60 g/l citramalate with a yield of 0.53 g citramalate/g glucose are generated in 132 hr Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + pyruvate + H2O
-
Escherichia coli CoA + (2R)-2-hydroxy-2-methylbutanedioate
-
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Synonyms

Synonyms Comment Organism
CimA
-
Escherichia coli