2.3.1.258: N-terminal methionine Nalpha-acetyltransferase NatE

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For detailed information about N-terminal methionine Nalpha-acetyltransferase NatE, go to the full flat file.

Word Map on EC 2.3.1.258

2.3.1.258

auxiliary

nt-acetylation

bisubstrate

n-acetyltransferase

n-termini

drug-induced

tunnel

chromatid

sister

acetylome

nalpha-terminal

co-translationally

medicine

Reaction

an N-terminal-L-methionyl-L-alanyl-[protein]

an N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein]

Synonyms

ARD1, EC 2.3.1.88, hNaa50, hNatA, MtRimI, N-terminal acetyltransferase E, NAA10, NAA15, Naa50, Naa50/San, Naa50p, Naa50p (NAT5/SAN) N-terminal acetyltransferase complex, Nalpha-acetyltransferase, NAT, NAT1, NAT5, NAT5/SAN, NatA, NatA/Naa50 complex, NatE, RimI, RimI acetyltransferase, Rv3420c, SAN, ScNaa50, ScNatA, SpNaa50, SpNatA

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.258 N-terminal methionine Nalpha-acetyltransferase NatE

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Results	in table
1	Activating Compound
33	AA Sequence
1	Application
13	Cloned(Commentary)
7	Cofactor
7	Crystallization (Commentary)
10	Disease/ Diagnostics
66	General Information
4	Inhibitors
2	kcat/KM [mM/s]
5	Ki Value [mM]
18	KM Value [mM]
14	Localization
1	Metals/Ions
2	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
46	Organism
1	Pathway
25	PDB ID
5	pH Optimum
36	Protein Variants
9	Purification (Commentary)
9	Reaction
30	Reference
20	Source Tissue
1	Specific Activity [micromol/min/mg]
65	Substrates and Products (Substrate)
11	Subunits
116	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.258 - N-terminal methionine Nalpha-acetyltransferase NatE

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HiTrap SP column chromatography, and Superdex 75 gel filtration

Homo sapiens

Q9GZZ1

719976

nickel affinity column chromatography

Homo sapiens

740753

recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity chromatography and gel filtration

Mycobacterium tuberculosis

I6YG32

758368

recombinant GST-tagged full-length Naa50p from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and cleavage of the GST-tag by TEV protease, followed by ion exchange chromatography and gel filtration

Homo sapiens

Q9GZZ1

719976

recombinant GST-tagged hNaa50 and hNatA fromSf9 insect cells by affinity chromatography and gel filtration

2 entries

recombinant GST-tagged SpNatA and SpNaa50 from Escherichia coli by glutathione affinity chromatography and gel filtration, SpNaa50 maintains the ability to co-migrate with SpNatA in sizing buffer with NaCl concentration as high as 1 M

Schizosaccharomyces pombe

758493

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration

Mycobacterium tuberculosis

I6YG32

755712

recombinant hNaa15 mutants, hNaa50, and hNatA by affinity chromatography and gel filtration

Homo sapiens

Q9GZZ1; P41227; Q9BXJ9

757754