2.3.1.258: N-terminal methionine Nalpha-acetyltransferase NatE

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For detailed information about N-terminal methionine Nalpha-acetyltransferase NatE, go to the full flat file.

Reaction

Synonyms

ARD1, EC 2.3.1.88, hNaa50, hNatA, MtRimI, N-terminal acetyltransferase E, NAA10, NAA15, Naa50, Naa50/San, Naa50p, Naa50p (NAT5/SAN) N-terminal acetyltransferase complex, Nalpha-acetyltransferase, NAT, NAT1, NAT5, NAT5/SAN, NatA, NatA/Naa50 complex, NatE, RimI, RimI acetyltransferase, Rv3420c, SAN, ScNaa50, ScNatA, SpNaa50, SpNatA

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.258 N-terminal methionine Nalpha-acetyltransferase NatE

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Results	in table
1	Activating Compound
33	AA Sequence
1	Application
13	Cloned(Commentary)
7	Cofactor
7	Crystallization (Commentary)
10	Disease/ Diagnostics
66	General Information
4	Inhibitors
2	kcat/KM [mM/s]
5	Ki Value [mM]
18	KM Value [mM]
14	Localization
1	Metals/Ions
2	Molecular Weight [Da]
13	Natural Substrates/ Products (Substrates)
46	Organism
1	Pathway
25	PDB ID
5	pH Optimum
36	Protein Variants
9	Purification (Commentary)
9	Reaction
30	Reference
20	Source Tissue
1	Specific Activity [micromol/min/mg]
65	Substrates and Products (Substrate)
11	Subunits
116	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
8	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.3.1.258 - N-terminal methionine Nalpha-acetyltransferase NatE

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

enzyme bound to a native substrate peptide fragment (MLGPEGGRWG) and CoA, hanging drop vapor diffusion method, using 16% (w/v) PEG 8000, 20% (v/v) glycerol, and 40 mM potassium phosphate (monobasic, pH 5.0)

Homo sapiens

Q9GZZ1

719976

hanging drop vapor diffusion method, using 20% (w/v) PEG 3350 and 0.2 M sodium formate

Homo sapiens

-

740753

Naa50p bound to a native substrate peptide fragment and CoA, hanging drop vapor diffusion method, mixing of 12 mg/ml protein in 25 mM HEPES, pH 7.5, 100 mM NaCl, and 10mM dithiothreitol, with peptide MLGPEGGRWG solution, and crystallization solution, containing 16% PEG 8000, 20% glycerol, and 40 mM potassium phosphate, pH 5.0, in a 1:3:3 molar ratio, 20°C, 1-3 days, X-ray diffraction structure determination and analysis at 2.75 A resolution

Homo sapiens

Q9GZZ1

719976

purified hNatE and hNatE in complex with inhibitor HYPK, X-ray diffraction structure determination and analysis at 4.5-5.0 A resolution

Homo sapiens

Q9GZZ1; P41227; Q9BXJ9

757754

NatA/Naa50 complex, i.e. NatE, including full-length ScNaa15 (residues 1-854), C-terminally truncated ScNaa10 (1-226 out of 238 total residues), and full-length ScNaa50 (residues 1-176), in the presence of inositol hexaphosphate (IP6) and bi-substrate analogues for both Naa10 and Naa50, X-ray diffraction structure determination and analysis

Saccharomyces cerevisiae

Q08689; P41227; P12945

758492

purified recombinant ScNatA/Naa50 complex, X-ray diffraction structure determination and analysis at 2.7 A resolution

Saccharomyces cerevisiae

Q08689; P07347; P12945

758493

NatA/Naa50 complex, i.e. NatE, X-ray diffraction structure determination and analysis

Schizosaccharomyces pombe

-

758492