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2.3.1.258: N-terminal methionine Nalpha-acetyltransferase NatE

This is an abbreviated version!
For detailed information about N-terminal methionine Nalpha-acetyltransferase NatE, go to the full flat file.

Word Map on EC 2.3.1.258

Reaction

acetyl-CoA
+
an N-terminal-L-methionyl-L-alanyl-[protein]
=
an N-terminal-Nalpha-acetyl-L-methionyl-L-alanyl-[protein]
+
CoA

Synonyms

ARD1, EC 2.3.1.88, hNaa50, hNatA, MtRimI, N-terminal acetyltransferase E, NAA10, NAA15, Naa50, Naa50/San, Naa50p, Naa50p (NAT5/SAN) N-terminal acetyltransferase complex, Nalpha-acetyltransferase, NAT, NAT1, NAT5, NAT5/SAN, NatA, NatA/Naa50 complex, NatE, RimI, RimI acetyltransferase, Rv3420c, SAN, ScNaa50, ScNatA, SpNaa50, SpNatA

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.258 N-terminal methionine Nalpha-acetyltransferase NatE

Cloned

Cloned on EC 2.3.1.258 - N-terminal methionine Nalpha-acetyltransferase NatE

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expression in Saccharomyces cerevisiae lacking endogenous Naa50
-
expression of GST-tagged full-length Naa50p with a TEV protease-cleavable site in Escherichia coli strain BL21(DE3)
gene rimI, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Rv3420c or rimI, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli
recombinant expression of hNaa15 mutants in HeLa cells, recombinant expression of N-terminally His-tagged NatA in Spodoptera frugiperda Sf9 cells, recombinant expression of His-tagged hNaa50 in Escherichia coli strain Rosetta (DE3)pLysS and strain BL21(DE3), coexpression of tagged HYPK
Q9GZZ1; P41227; Q9BXJ9
recombinant expression ofthe NatA/Naa50 complex, i.e. NatE, in Escherichia coli
Q08689; P41227; P12945
recombinant overexpression of GST-tagged hNaa50 and hNatA in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
recombinant overexpression of N-terminally GST-tagged SpNatA and SpNaa50 in Escherichia coli
-