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1.14.15.24: beta-carotene 3-hydroxylase

This is an abbreviated version!
For detailed information about beta-carotene 3-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.24

Reaction

beta-carotene
+ 2 reduced ferredoxin [iron-sulfur] cluster +
H+
+
O2
=
beta-cryptoxanthin
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O

Synonyms

AtB1, AtB2, BCH, BCH-type carotene hydroxylase, BCH1, BCH2, beta-carotene (beta,beta-carotene) 3-hydrocylase, beta-carotene 3,3'-hydroxylase, beta-carotene 3-hydroxylase, beta-carotene hydroxylase, beta-carotene hydroxylase 2, beta-carotene oxygenase, beta-hydroxylase, beta-hydroxylase (b1,b2), beta-hydroxylases 1, beta-hydroxylases 2, beta-ring 3(3')-hydroxylase, betaOHase, Bhy, carotene beta hydroxylase 2, carotene ring hydroxylase, carotenoid (beta-ionone ring) 3,3'-hydroxylase, carotenoid 3,(3')-beta-ionone ring hydroxylase, carotenoid 3,3'-hydroxylase, carotenoid beta-ring hydroxylase, carotenoid hydroxylase, carotenoid hydroxylase 2, Chx, CHX1, CHX2, Chy, CHY-beta, CHY1, CHY1/2, CHY2, Chyb, CrtH1, CrtR-B, CrtR-b2, crtRB1, crtS, CrtZ, CrtZ-2, CYP175A1, CYP97A, CYP97a3, CYP97A4, CYP97C1, DSM2, EC 1.14.13.129, HYD, L-carotene hydroxylase, LUT1, LUT5

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.24 beta-carotene 3-hydroxylase

Purification

Purification on EC 1.14.15.24 - beta-carotene 3-hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and hydroxyapatite column chromatography
-
Ni-NTA column chromatography
Q-Sepharose column chromatography, Talon nickel-affinity resin column chromatography, and Mono Q column chromatography