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1.14.15.24: beta-carotene 3-hydroxylase

This is an abbreviated version!
For detailed information about beta-carotene 3-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.24

Reaction

beta-carotene
+ 2 reduced ferredoxin [iron-sulfur] cluster +
H+
 +
O2
=
beta-cryptoxanthin
 + 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O

Synonyms

AtB1, AtB2, BCH, BCH-type carotene hydroxylase, BCH1, BCH2, beta-carotene (beta,beta-carotene) 3-hydrocylase, beta-carotene 3,3'-hydroxylase, beta-carotene 3-hydroxylase, beta-carotene hydroxylase, beta-carotene hydroxylase 2, beta-carotene oxygenase, beta-hydroxylase, beta-hydroxylase (b1,b2), beta-hydroxylases 1, beta-hydroxylases 2, beta-ring 3(3')-hydroxylase, betaOHase, Bhy, carotene beta hydroxylase 2, carotene ring hydroxylase, carotenoid (beta-ionone ring) 3,3'-hydroxylase, carotenoid 3,(3')-beta-ionone ring hydroxylase, carotenoid 3,3'-hydroxylase, carotenoid beta-ring hydroxylase, carotenoid hydroxylase, carotenoid hydroxylase 2, Chx, CHX1, CHX2, Chy, CHY-beta, CHY1, CHY1/2, CHY2, Chyb, CrtH1, CrtR-B, CrtR-b2, crtRB1, crtS, CrtZ, CrtZ-2, CYP175A1, CYP97A, CYP97a3, CYP97A4, CYP97C1, DSM2, EC 1.14.13.129, HYD, L-carotene hydroxylase, LUT1, LUT5

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.24 beta-carotene 3-hydroxylase

Cloned

Cloned on EC 1.14.15.24 - beta-carotene 3-hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
astaxanthin biosynthesis pathway is constructed in Saccharomyces cerevisiae by introducing heterologous CrtZ and CrtW into an existing high beta-carotene producing strain. Both genes crtZ and crtW are codon optimized and expressed under the control of constitutive promoters. Strain SyBE_Sc118060 with CrtW from Brevundimonas vesicularis DC263 and CrtZ from Alcaligenes sp. strain PC-1 achieves the highest astaxanthin yield of 3.1 mg/g dry cell weight
expressed in calli of Ipomoea batatas cultivar Yulmi
expressed in Corynebacterium glutamicum
-
expressed in Dunaliella salina
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH10BZIP cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli JM101 cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli strain JM101 and in Arabidopsis thaliana under the control of the seed-speciWc napin promoter from Brassica napus
-
expressed in Kluyveromyces marxianus strain KY3
expressed in Mucor circinelloides strain MS12
-
expressed in Nicotiana tabacum
-
expressed in Nicotiana tabacum cultivar Nipponbare
-
expressed in Nicotiana tabacum cultivar SR-1
expressed in Saccharomyces cerevisiae
expression in Escherichia coli
expression of the crtW and crtZ from DC263 in a beta-carotene-accumulating Escherichia coli produces astaxanthin as the predominant carotenoid
overexpression in Sacharomyces cerecisiae YastD-01
the genes coding for CrtW and CrtZ from Brevundimonas sp. SD212 are introduced into Nicotiana tabacum by transplastomic engineering (plastid-transformation engineering) and expressed successfully