EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.45 | I162T |
specific activity is 1.9fold higher than that of wild-type |
764955 |
| 1.14.11.45 | I162T/T182N |
double mutant with improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively |
764955 |
| 1.14.11.45 | L27I/E80D/G169H/S182D |
catalytic efficiency, thermal stability and catalytic rate are significantly improved compared with those of wild-type IDO |
764247 |
| 1.14.11.45 | more |
mutants with improved activity are obtained by a strategy dependent on the restoration of succinate-minus Escherichia coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of 2-oxoglutarate to succinate |
764247 |
| 1.14.11.45 | N126H |
mutant obtained by directed evolution, 2.8fold increase in activity |
764363 |
| 1.14.11.45 | N126H/T130K |
catalytic efficiency is 2.4fold and 3.0fold higher than wild-type with Ile and 2-oxoglutaric acid as substrates |
764363 |
| 1.14.11.45 | T130K |
mutant obtained by site-specific mutagenesis, 1.7fold increase in activity |
764363 |
| 1.14.11.45 | T182N |
mutant achieves up to 70% conversion of Ile |
764955 |
