Application | Comment | Organism |
---|---|---|
synthesis | development of efficient method for the biotransformation of 4-hydroxyisoleucine by resting cells expressing L27I/E80D/G169H/S182D 151.9 mM of 4-hydroxyisoleucine/l (22.4 g/l) can be synthesized in 12 h while the substrates seldom exhibits additional consumption | Bacillus thuringiensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus thuringiensis |
Protein Variants | Comment | Organism |
---|---|---|
L27I/E80D/G169H/S182D | catalytic efficiency, thermal stability and catalytic rate are significantly improved compared with those of wild-type IDO | Bacillus thuringiensis |
additional information | mutants with improved activity are obtained by a strategy dependent on the restoration of succinate-minus Escherichia coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of 2-oxoglutarate to succinate | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
L-isoleucine | mutant L27I/E80D/G169H/S182D, pH 7.0, 30°C | Bacillus thuringiensis | |
0.2 | - |
L-isoleucine | wild-type, pH 7.0, 30°C | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | S5SPJ1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, more than 60% residual activity | Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
L-isoleucine | wild-type, pH 7.0, 30°C | Bacillus thuringiensis | |
0.084 | - |
L-isoleucine | mutant L27I/E80D/G169H/S182D, pH 7.0, 30°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
L-isoleucine | wild-type, pH 7.0, 30°C | Bacillus thuringiensis | |
0.56 | - |
L-isoleucine | mutant L27I/E80D/G169H/S182D, pH 7.0, 30°C | Bacillus thuringiensis |