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Literature summary for 1.14.11.45 extracted from

  • Du, P.; Yan, S.; Qian, X.L.; Pan, J.; Zhang, Z.J.; Yu, H.L.; Xu, J.H.
    Engineering Bacillus subtilis isoleucine dioxygenase for efficient synthesis of (2S,3R,4S)-4-hydroxyisoleucine (2020), J. Agric. Food Chem., 68, 14555-14563 .
    View publication on PubMed
Search for more literature for 1.14.11.45

Application

Application Comment Organism
synthesis double mutant I162T/T182N shows improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively. L-Isoleucine (228 mM) is completely converted to (2S,3R,4S)-4-HIL with a space-time yield of up to 80.8 g/l and d. With a increase of the substrate loading to 1 M, a high conversion of 91% can also be achieved Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
I162T specific activity is 1.9fold higher than that of wild-type Bacillus subtilis
I162T/T182N double mutant with improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively Bacillus subtilis
T182N mutant achieves up to 70% conversion of Ile Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 mutant T182N, pH 7.0, 30°C Bacillus subtilis
3.8
-
 wild-type, pH 7.0, 30°C Bacillus subtilis
6.4
-
 mutant I162T, pH 7.0, 30°C Bacillus subtilis
12
-
 mutant I162T/T182N, pH 7.0, 30°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2-oxoglutarate + O2
-
 Bacillus subtilis (4S)-4-hydroxy-L-isoleucine + succinate + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus subtilis