EC Number |
Protein Variants |
Reference |
---|
1.13.11.27 | A327R/L359M |
about 90% of wild-type activity |
765506 |
1.13.11.27 | E12A |
2fold decrease in catalytic efficiency |
765020 |
1.13.11.27 | E254D |
site-directed mutagenesis, the mutant shows 5% remaining activity compared to the wild-type enzyme |
726347 |
1.13.11.27 | E322R |
mutant shows improved resistance to topramezone, with about 98% of wild-type activity, and displays increased resistance to mesotrione |
764613 |
1.13.11.27 | F337I |
0.67 of wild-type activity |
658081 |
1.13.11.27 | F341Y |
16.9% of wild-type activity |
658081 |
1.13.11.27 | F364I |
site-directed mutagenesis, the mutant enzyme produces 47% homogentisate, 15% 4-hydroxyphenylacetate, and 19% quinolacetate, which differs from the wild-type activity |
724392 |
1.13.11.27 | F381A |
residue is involved in substrate binding |
765756 |
1.13.11.27 | G411A |
about 15% of wild-type activity |
765506 |
1.13.11.27 | G413W |
about 20% of wild-type activity |
765506 |