Protein Variants | Comment | Organism |
---|---|---|
E12A | 2fold decrease in catalytic efficiency | Homo sapiens |
K10A | 2fold decrease in catalytic efficiency | Homo sapiens |
additional information | upon removal of the 12 N-terminal residues, the kcat/Km decreases 8fold compared to wild-type. The wild-type level of activity is retained in a mutant missing the 17 N-terminal residues, with a kcat/Km 11fold higher than that of the mutant lacking 12 N-terminal residues, however, the structural stability of this mutant is lower than that of wild type | Homo sapiens |
P11A | 30% increase in catalytic efficiency | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-hydroxyphenylpyruvate | mutant K10A, pH 6.5, 37°C | Homo sapiens | |
0.1 | - |
4-hydroxyphenylpyruvate | mutant lacking 17 N-terminal residues, pH 6.5, 37°C | Homo sapiens | |
0.18 | - |
4-hydroxyphenylpyruvate | mutant E12A, pH 6.5, 37°C | Homo sapiens | |
0.18 | - |
4-hydroxyphenylpyruvate | mutant P11A, pH 6.5, 37°C | Homo sapiens | |
0.24 | - |
4-hydroxyphenylpyruvate | wild-type, pH 6.5, 37°C | Homo sapiens | |
0.37 | - |
4-hydroxyphenylpyruvate | mutant lacking 12 N-terminal residues, pH 6.5, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P32754 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Homo sapiens | homogentisate + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54.4 | - |
melting temperature, mutant lacking 17 N-terminal residues | Homo sapiens |
56.5 | - |
melting temperature, mutant lacking 12 N-terminal residues | Homo sapiens |
56.7 | - |
melting temperature, mutant E12A | Homo sapiens |
57.1 | - |
melting temperature, wild-type | Homo sapiens |
58 | - |
melting temperature, mutant P11A | Homo sapiens |
59.8 | - |
melting temperature, mutant K10A | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-hydroxyphenylpyruvate | mutant K10A, pH 6.5, 37°C | Homo sapiens | |
0.7 | - |
4-hydroxyphenylpyruvate | mutant lacking 12 N-terminal residues, pH 6.5, 37°C | Homo sapiens | |
1.4 | - |
4-hydroxyphenylpyruvate | mutant E12A, pH 6.5, 37°C | Homo sapiens | |
2.2 | - |
4-hydroxyphenylpyruvate | mutant lacking 17 N-terminal residues, pH 6.5, 37°C | Homo sapiens | |
3.5 | - |
4-hydroxyphenylpyruvate | mutant P11A, pH 6.5, 37°C | Homo sapiens | |
3.7 | - |
4-hydroxyphenylpyruvate | wild-type, pH 6.5, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-hydroxyphenylpyruvate | mutant lacking 12 N-terminal residues, pH 6.5, 37°C | Homo sapiens | |
8 | - |
4-hydroxyphenylpyruvate | mutant E12A, pH 6.5, 37°C | Homo sapiens | |
8 | - |
4-hydroxyphenylpyruvate | mutant K10A, pH 6.5, 37°C | Homo sapiens | |
15 | - |
4-hydroxyphenylpyruvate | wild-type, pH 6.5, 37°C | Homo sapiens | |
20 | - |
4-hydroxyphenylpyruvate | mutant P11A, pH 6.5, 37°C | Homo sapiens | |
22 | - |
4-hydroxyphenylpyruvate | mutant lacking 17 N-terminal residues, pH 6.5, 37°C | Homo sapiens |