2.4.1.214: glycoprotein 3-alpha-L-fucosyltransferase
This is an abbreviated version!
For detailed information about glycoprotein 3-alpha-L-fucosyltransferase, go to the full flat file.
Reaction
+
=
+
Synonyms
alpha 1,3-fucosyltransferase VI, alpha(1,3)-fucosyltransferase VII, alpha(1,3)-fucosyltransferase-IV, alpha(1,3)-fucosyltransferase-VII, alpha(1,3)fucosyltransferase, alpha-1,3-fucosyltransferase-VII, alpha1,3-fucosyltransferase, alpha1,3-fucosyltransferase 10, alpha1,3-fucosyltransferase 7, alpha1,3-fucosyltransferase 9, alpha1,3-fucosyltransferase IX, alpha1,3FTs, alpha1-3 fucosyltransferase-VII, alpha3-fucosyltransferase, alpha3-FucT VII, AtFUT11, CEFT-1, CEFT-2, CEFT-3, CEFT-4, CEFT-5, CEFT3, core alpha-(1,3)-fucosyltransferase, core alpha1,3-fucosyltransferase, Fuc-T C3, Fuc-TVII, fucosyltransferase A, fucosyltransferase VII, FucT, FucT 115, FucT 45, FucT VI, FucT VII, FucT-IV, FucT-VII, FucT1, FucTA, FucTB, FucTC, FUT-1, FUT-6, FUT10, FUT11, FUT7, Fut9, GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fuca1-6GlcNAc)-Asn-peptide) alpha1-3-fucosyltransferase, GDP-L-fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase, GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase, LOC107797347, LOC107830046, NM_001324945, XM_016620229, XM_016657530
ECTree
KM Value
KM Value on EC 2.4.1.214 - glycoprotein 3-alpha-L-fucosyltransferase
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0.49
bisected agalacto-glycopeptide-IgGF6
-
-
-
0.046 - 0.067
dansyl-GnGn
0.011
dansyl-GnGnF6
-
glycopeptide derived from IgG, FucTA gene
0.5
fetuin
-
recombinant soluble enzyme
-
0.0062
GDP-alpha-L-fucose
-
recombinant soluble enzyme
0.1 - 1
GDP-beta-L-fucose
0.193
GDP-L-Fuc
pH 6.0, 37°C
0.019 - 0.336
GDP-L-fucose
0.838
GDP-L-Gal
pH 6.0, 37°C
6.7
GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(ucalpha(1->6)G)cNAcbeta(1->)N-Asn-peptide(NAc)
-
GnGn + F(Ac)
-
0.42
GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
at 25°C, pH not specified in the publication
1
GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
at 25°C, pH not specified in the publication
0.06 - 0.13
GnGnF6-peptide
0.71 - 12.77
N-acetyllactosamine
5.6
NeuAcalpha2-3Galbeta1-4GlcNAc
-
recombinant soluble enzyme
additional information
additional information
-
kinetics of wild-type and alpha 1,3 FucT motif mutants
-
0.046
dansyl-GnGn
-
glycopeptide derived from IgG, FucTA gene
0.067
dansyl-GnGn
-
FucTA gene
0.1
GDP-beta-L-fucose
mutant enzyme S253A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C
0.24
GDP-beta-L-fucose
mutant enzyme Y243A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C
0.33
GDP-beta-L-fucose
mutant enzyme N219A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C
0.36
GDP-beta-L-fucose
mutant enzyme T339A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C
1
GDP-beta-L-fucose
wild type enzyme, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C
0.065
GDP-fucose
-
-
0.0683
GDP-fucose
deletion mutant FTdelta23 with 23 residues deleted from the C-terminus
0.07864
GDP-fucose
deletion mutant FTdelta66 with 66 residues deleted from the C-terminus
0.07967
GDP-fucose
deletion mutant FTdelta38 with 38 residues deleted from the C-terminus
0.08016
GDP-fucose
-
FucT 45, N240A mutant
0.08831
GDP-fucose
deletion mutant FTdelta45 with 45 residues deleted from the C-terminus
0.08831
GDP-fucose
-
FucT 45
0.09356
GDP-fucose
deletion mutant FTdelta28 with 28 residues deleted from the C-terminus
0.09864
GDP-fucose
deletion mutant FTdelta80 with 80 residues deleted from the C-terminus
0.157
GDP-fucose
-
FucT 45, Y246F mutant
0.2223
GDP-fucose
-
FucT 115
0.228
GDP-fucose
deletion mutant FTdelta115 with 115 residues deleted from the C-terminus
0.59 - 1
GDP-fucose
-
FucT 45, R195K mutant
0.8
GDP-fucose
-
FucT 45, Y246A mutant
0.019
GDP-L-fucose
-
pH 6.5, recombinant wild-type FucT VI
0.069
GDP-L-fucose
-
pH 6.5, recombinant FucT VI mutant S249A
0.11
GDP-L-fucose
-
pH 6.5, recombinant FucT VI mutant F246A
0.22
GDP-L-fucose
-
pH 6.5, recombinant FucT VI mutant Y240A
0.336
GDP-L-fucose
-
pH 6.5, recombinant FucT VI mutant L244A
0.19
GnGn-peptide
-
-
0.06
GnGnF6-peptide
-
-
0.7
LacNAc
deletion mutant FTdelta38 with 38 residues deleted from the C-terminus
0.71
LacNAc
deletion mutant FTdelta45 with 45 residues deleted from the C-terminus
0.71
LacNAc
deletion mutant FTdelta66 with 66 residues deleted from the C-terminus
0.78
LacNAc
deletion mutant FTdelta23 with 23 residues deleted from the C-terminus
0.78
LacNAc
deletion mutant FTdelta28 with 28 residues deleted from the C-terminus
0.89
LacNAc
deletion mutant FTdelta80 with 80 residues deleted from the C-terminus
8.92
LacNAc
deletion mutant FTdelta115 with 115 residues deleted from the C-terminus
0.13
LacNAc-C8
-
pH 6.5, recombinant FucT VI mutants Y240A and L244A
0.15
LacNAc-C8
-
pH 6.5, recombinant FucT VI mutant F246A
0.22
LacNAc-C8
-
pH 6.5, recombinant wild-type FucT VI
1.6
LacNAc-C8
-
pH 6.5, recombinant FucT VI mutant S249A
0.71
N-acetyllactosamine
-
FucT 45
1.29
N-acetyllactosamine
-
FucT 45, Y246F mutant
2.39
N-acetyllactosamine
-
FucT 45, Y246A mutant
8.92
N-acetyllactosamine
-
FucT 115
10.35
N-acetyllactosamine
-
FucT 45, R195K mutant
12.77
N-acetyllactosamine
-
FucT 45, N240A mutant