Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
A642N
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
K639R
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
L636Q/K639R/V641R/A642N
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
L636Q/L637M/K639R/T640V/V641R/A642N
the mutant exhibits a strongly decreased Km value for UDP and increased Km for ADP as compared to the wild type enzyme
L636Q/V641R/A642N
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
L637M
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
L637M/K639R/T640V
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
T640V
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
A642N
-
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
-
K639R
-
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
-
L636Q/V641R/A642N
-
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
-
L637M
-
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
-
T640V
-
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
-
S11A
phosphorylation still occurs, but weakly
S11C
phosphorylation still occurs, but weakly
S11D
phosphorylation still occurs, but weakly
SUS1-DELTA9
-
the kcat/Km of the mutant toward sucrose isdecreased by 28.4% compared to that of wild type isoform SUS1, while the kcat/Km of the mutant toward UDP is increased by 1.6% compared to that of SUS1
SUS2-DELTA9
-
the kcat/Km of the mutant toward UDP is increased by 3.8fold compared to that of wild type isoform SUS2, while the kcat/Km of the mutant toward sucrose is decreased by 35.7% compared to that of SUS2
E678D
site-directed mutagenesis, almost inactive mutant
E678Q
site-directed mutagenesis, almost inactive mutant, the mutant shows impaired D-fructose binding
E686D
site-directed mutagenesis, the mutant enzyme retains 34.9% of sucrose cleavage and 37.9% of sucrose synthesis activity, respectively
E686Q
site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding
F680S
site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding
F680Y
site-directed mutagenesis, the mutant enzyme retains 61.0% of sucrose cleavage and all of sucrose synthesis activity, respectively
S11A
site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Saccharomyces cerevisiae
S11D
site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Escherichia coli
L636Q/A642N
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
L636Q/A642N
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
L637M/T640V
the double mutant shows a 60fold reduced KM value for UDP and substantially improved UDP-glucose recycling. The mutant is roughly 8times more active than the wild type enzyme
L637M/T640V
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
L636Q/A642N
-
site-directed mutagenesis, the mutant shows unaltered Km for UDP compared to the wild-type enzyme
-
L636Q/A642N
-
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
-
L637M/T640V
-
the double mutant shows a 60fold reduced KM value for UDP and substantially improved UDP-glucose recycling. The mutant is roughly 8times more active than the wild type enzyme
-
L637M/T640V
-
the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme
-
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation and analysis of isozyme mutant lines
additional information
generation and analysis of isozyme mutant lines
additional information
generation and analysis of isozyme mutant lines
additional information
generation and analysis of isozyme mutant lines
additional information
generation and analysis of isozyme mutant lines
additional information
generation and analysis of isozyme mutant lines
additional information
-
generation and analysis of isozyme mutant lines
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
manipulation of sugar levels in transgenic plants by overexpressing sucrose synthase
additional information
-
gene sus5 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
-
additional information
-
generation of a sus1 mutant and a sus1/sus4 double mutant. None of the other five genes display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
-
additional information
-
gene SUS2 does not display an altered pattern of expression as a consequence of the lack of SUS1
-
additional information
-
gene sus6 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
-
additional information
-
generation of a sus4 mutant and a sus1/sus4 double mutant. Gene sus4 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
-
additional information
-
gene SUS3 does not display an altered pattern of expression as a consequence of the lack of SUS1. Impact of SUS mutations on ethanol production and ATP/ADP ratio under low-oxygen conditions, overview
-
additional information
-
activity and specific activity of sucrose synthase in podwalls are significantly higher in genetically modified temperature-tolerant lines 5012, 5014 and 5039 compared to wild-type ICCV 96029 and 96030, overview
additional information
antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance. Compared with the wild-type cucumbers, the transgenic lines with antisense expression of CsSUS3 are more sensitive to flooding. After 6 days of flooding, the enzyme activity, soluble sugar and UDP-glucose content and the ratio of ATP/ADP in the roots of transgenic plants are significantly lower than that in wild-type plants. The transgenic lines grow more slowly with more yellow necrosis in the leaves, phenotype overview
additional information
-
antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance. Compared with the wild-type cucumbers, the transgenic lines with antisense expression of CsSUS3 are more sensitive to flooding. After 6 days of flooding, the enzyme activity, soluble sugar and UDP-glucose content and the ratio of ATP/ADP in the roots of transgenic plants are significantly lower than that in wild-type plants. The transgenic lines grow more slowly with more yellow necrosis in the leaves, phenotype overview
additional information
-
antisense suppression of Cucumis sativus sucrose synthase 3 reduces hypoxic stress tolerance. Compared with the wild-type cucumbers, the transgenic lines with antisense expression of CsSUS3 are more sensitive to flooding. After 6 days of flooding, the enzyme activity, soluble sugar and UDP-glucose content and the ratio of ATP/ADP in the roots of transgenic plants are significantly lower than that in wild-type plants. The transgenic lines grow more slowly with more yellow necrosis in the leaves, phenotype overview
-
additional information
N-terminal truncation, phosphorylations still occurs, but weakly
additional information
-
N-terminal truncation, phosphorylations still occurs, but weakly
additional information
-
silencing of Sus expression in the endosperm by RNAi disrupts its cellularisation process and inhibits early seed development
additional information
-
construction of trangenic antisense construct expressing plants, that display up to 10fold reduced SucS1 levels in roots anf reduced arbuscular mycorrhiza. Mycorrhizal MtSucS1-reduced lines exhibit an overall stunted aboveground growth under phosphorus limitation. The transgenic plants show, apart from a reduced plant height, shoot weight, and leaf development, a delayed flowering, resulting in a lower seed yield. In addition, the root-to-shoot and root weight ratios increase significantly. Major reversion of arbuscular mycorrhiza-associated transcription, exhibiting a significant repression of well-known plant arbuscular mycorrhiza marker and mycosymbiont genes, together indicating a diminished AM fungus colonization of MtSucS1-antisense lines, phenotype, overview
additional information
-
overexpression of the Gossypium hirsutum SuSy gene under control of two promoters in hybrid poplar Populus alba x grandidentata leads to significantly increased SuSy enzyme activity in developing xylem and to increased secondary cell wall cellulose content
additional information
-
root restriction treatment increases the enzyme expression, while it reduces cytochrome pathway, and alternative pathway respirations with reduced ATP content. The ratio of invertase/sucrose synthase activity is increased in the restricted roots together with a decrease in glucose content and an increase in fructose content
additional information
-
construction of transgenic enzyme overexpressing plants and of transgenic antisense plants. ADPG diphosphorylase and UDPG diphosphorylase activities in tubers from overexpressing plants are significantly higher than in control tubers, whereas ADPG diphosphorylase activities in tubers of one SuSy line are significantly lower than in control tubers
additional information
-
recombinant ectopic expression of the enzyme from Solanum tuberosum in Zea mays seed endosperm leads to transgenic developing seeds that exhibit a significant increase in SuSy activity. The transgenic seeds accumulate 10-15% more starch at the mature stage and contain a higher amylose/amylopectin balance than wild-type maize seeds, while no significant changes are detected in the transgenic seeds in the content of soluble sugars, and in activities of starch metabolism-related enzymes when compared with wild-type seeds, phenotype, overview. Enhancement of the enzyme activity does not affect the redox status of AGP