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additional information
additional information
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0.0039
sucrose
30°C, pH 5.0, wild-type enzyme
0.0267
sucrose
-
sucrose hydrolysis, mutant R331S
0.117
sucrose
-
sucrose hydrolysis, mutant R331L
0.367
sucrose
-
sucrose hydrolysis, mutant R331H
0.583
sucrose
-
sucrose hydrolysis, wild-type
1
sucrose
wild-type enzyme
1.47
sucrose
-
sucrose hydrolysis, mutant R331K
1.7
sucrose
30°C, pH 5.0, mutant enzyme D135N
6.3
sucrose
mutant enzyme Y429N
6.4
sucrose
mutant enzyme S164A
7.2
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
13.3
sucrose
mutant D309N
13.5
sucrose
mutant enzyme R360S
28.3
sucrose
-
wild-type enzyme
41.8
sucrose
-
mutant enzyme K372A, hydrolytic activity, at pH 6.0 and 50°C
57.4
sucrose
mutant enzyme G361F
58.24
sucrose
-
mutant enzyme N251W/K372Y, transfructosylation activity, at pH 6.0 and 50°C
66.9
sucrose
30°C, pH 5.0, mutant enzyme C339S
71.8
sucrose
30°C, pH 5.0, mutant enzyme C395S
81.77
sucrose
-
mutant enzyme N251W, transfructosylation activity, at pH 6.0 and 50°C
87.5
sucrose
mutant enzyme R433A
99.69
sucrose
-
mutant enzyme K372A, transfructosylation activity, at pH 6.0 and 50°C
108
sucrose
-
levansucrase-endolevanase fusion enzyme, pH and temperature not specified in the publication
141.5
sucrose
mutant enzyme H243L
144.68
sucrose
-
mutant enzyme K372Y, hydrolytic activity, at pH 6.0 and 50°C
156.86
sucrose
-
mutant enzyme K372A, total activity, at pH 6.0 and 50°C
164
sucrose
-
levansucrase, pH and temperature not specified in the publication
164.6
sucrose
wild-type enzyme
166.3
sucrose
mutant enzyme I341V
170.2
sucrose
mutant enzyme R369K
207.11
sucrose
-
wild type enzyme, hydrolytic activity, at pH 6.0 and 50°C
214.01
sucrose
-
mutant enzyme K372Y, transfructosylation activity, at pH 6.0 and 50°C
220.35
sucrose
-
mutant enzyme N251W/K372Y, total activity, at pH 6.0 and 50°C
325
sucrose
-
pH 6.6, mutant enzyme Y421A
326.62
sucrose
-
mutant enzyme K372Y, total activity, at pH 6.0 and 50°C
328.3
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
335
sucrose
-
mutant enzyme Y421A
363
sucrose
-
pH 6.6, mutant enzyme S173A
363
sucrose
-
mutant enzyme S173A
373.22
sucrose
-
wild type enzyme, total activity, at pH 6.0 and 50°C
379
sucrose
Q60114
transfructosylation activity
411
sucrose
Q60114
levan synthesis activity
461
sucrose
Q60114
total activity
497.9
sucrose
at pH 6.5 and 37°C
502.7
sucrose
-
at pH 6.5 and 37°C
504.4
sucrose
sucrose hydrolysis, pH 6.0-6.6, 60°C
672.38
sucrose
-
wild type enzyme, transfructosylation activity, at pH 6.0 and 50°C
758.78
sucrose
-
mutant enzyme N251W, hydrolytic activity, at pH 6.0 and 50°C
1000
sucrose
-
pH 6.6, mutant enzyme W94A
1000
sucrose
-
mutant enzyme W94A
1118.63
sucrose
-
mutant enzyme N251W, total activity, at pH 6.0 and 50°C
1231
sucrose
-
pH 6.6, mutant enzyme L118A
1231
sucrose
-
mutant enzyme L118A
1480
sucrose
-
pH 6.6, mutant enzyme N252A
1480
sucrose
-
mutant enzyme N252A
1500
sucrose
transfructosylating activity, at pH 6.0 and 37°C
1529
sucrose
-
mutant enzyme N252H
2256
sucrose
-
mutant enzyme N252G
2272
sucrose
-
pH 6.6, wild-type enzyme
2396
sucrose
-
pH 6.6, mutant enzyme W172A
2396
sucrose
-
mutant enzyme W172A
3743
sucrose
-
mutant enzyme N252D
4700
sucrose
transfructosylating activity, at pH 6.0 and 37°C
5700
sucrose
hydrolytic activity, at pH 6.0 and 37°C
7200
sucrose
total activity, at pH 6.0 and 37°C
8000
sucrose
hydrolytic activity, at pH 6.0 and 37°C
9000
sucrose
transfructosylating activity, at pH 6.0 and 37°C
12000
sucrose
total activity, at pH 6.0 and 37°C
19300
sucrose
hydrolytic activity, at pH 6.0 and 37°C
33600
sucrose
transfructosylating activity, at pH 6.0 and 37°C
55400
sucrose
total activity, at pH 6.0 and 37°C
100000
sucrose
transfructosylating activity, at pH 6.0 and 37°C
157300
sucrose
transfructosylating activity, at pH 6.0 and 37°C
176000
sucrose
hydrolytic activity, at pH 6.0 and 37°C
200000
sucrose
hydrolytic activity, at pH 6.0 and 37°C
200000
sucrose
total activity, at pH 6.0 and 37°C
300000
sucrose
hydrolytic activity, at pH 6.0 and 37°C
300000
sucrose
transfructosylating activity, at pH 6.0 and 37°C
317400
sucrose
total activity, at pH 6.0 and 37°C
600000
sucrose
total activity, at pH 6.0 and 37°C
additional information
additional information
-
mutants, overview
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additional information
additional information
mutants A344P and F414W have the same behavior like the wild-type
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additional information
additional information
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mutants A344P and F414W have the same behavior like the wild-type
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