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evolution
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the enzyme belongs to the glycoside hydrolase family GH68
evolution
the enzyme shows the five-bladed beta-propeller typical of glycoside hydrolase families 32 and 68 members
evolution
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the enzyme belongs to the glycoside hydrolase family GH68
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evolution
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the enzyme shows the five-bladed beta-propeller typical of glycoside hydrolase families 32 and 68 members
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malfunction
a hexR mutant of PG4180 is significantly growth-impaired when incubated with sucrose or glucose as sole carbon source, but exhibited wild type growth when arabinose is provided, higher transcript and protein levels of the enzyme occur in the hexR mutant as compared to the wild-type
malfunction
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a hexR mutant of PG4180 is significantly growth-impaired when incubated with sucrose or glucose as sole carbon source, but exhibited wild type growth when arabinose is provided, higher transcript and protein levels of the enzyme occur in the hexR mutant as compared to the wild-type
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metabolism
bacterial levansucrases polymerize fructose residues of sucrose to beta-2,6 linked fructans-fructooligosaccharides (FOS) and levan
metabolism
coregulation of genes involved in extracellular sugar acquisition with those involved in intracellular energy-providing metabolic pathways in Pseudomonas syringae
metabolism
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the enzyme is a key biocatalyst in the synthesis of levan and levan-type fructooligosaccharides
metabolism
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the enzyme is a key biocatalyst in the synthesis of levan and levan-type fructooligosaccharides
metabolism
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the enzyme is a key biocatalyst in the synthesis of levan and levan-type fructooligosaccharides
metabolism
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coregulation of genes involved in extracellular sugar acquisition with those involved in intracellular energy-providing metabolic pathways in Pseudomonas syringae
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metabolism
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bacterial levansucrases polymerize fructose residues of sucrose to beta-2,6 linked fructans-fructooligosaccharides (FOS) and levan
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physiological function
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fructan accumulation occurs mainly in the first 2 weeks after anthesis, and a maximal fructan concentration of 2.5 mg fructan per kernel is reached at 16 days after anthesis. Fructan synthesis is catalyzed by 1-SST (sucrose:sucrose 1-fructosyltransferase) and 6-SFT (sucrose:fructan 6-fructosyltransferase), and to a lesser extent by 1-FFT (fructan:fructan 1-fructosyltransferase). Despite the presence of 6G-kestotriose in wheat kernel extracts, the measured 6G-FFT (fructan:fructan 6G-fructosyltransferase) activity levels are low
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high. Enzyme LsdA is specifically prone for fructooligosaccharide synthesis, producing inulin-type fructooligosaccharides and only a small amount of levan
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high. The plant enzyme synthesizes linear beta-2,6-linked fructans which are referred to as plant levans or phleins. Phleins have lower degree of polymerization than bacterial levans which usually have polymers of DP over 100
physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high. The plant enzyme synthesizes linear beta-2,6-linked fructans which are referred to as plant levans or phleins. Phleins have lower degree of polymerization than bacterial levans which usually have polymers of DP over 100
physiological function
levan is produced by a single enzyme, levansucrase. Levan is required for the formation of a protective biofilm and represents one of several virulence factors of the bacterium
physiological function
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Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity stimulating IgA production in mice
physiological function
plant sucrose is utilized by invading bacteria via the secreted enzyme, levansucrase, liberating glucose and forming the polyfructan levan. Transcription of lsc is controlled by the hexose metabolism repressor, HexR
physiological function
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transfructosylation reaction is an important method for producing lactosucrose, in which sucrose and lactose serve as the fructosyl donor and acceptor, respectively
physiological function
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transfructosylation reaction is an important method for producing lactosucrose, in which sucrose and lactose serve as the fructosyl donor and acceptor, respectively
physiological function
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transfructosylation reaction is an important method for producing lactosucrose, in which sucrose and lactose serve as the fructosyl donor and acceptor, respectively
physiological function
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plant sucrose is utilized by invading bacteria via the secreted enzyme, levansucrase, liberating glucose and forming the polyfructan levan. Transcription of lsc is controlled by the hexose metabolism repressor, HexR
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physiological function
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Levansucrase from Leuconostoc mesenteroides NTM048 produces a levan exopolysaccharide with immunomodulating activity stimulating IgA production in mice
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physiological function
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in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high
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physiological function
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levan is produced by a single enzyme, levansucrase. Levan is required for the formation of a protective biofilm and represents one of several virulence factors of the bacterium
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additional information
enzyme structure comparisons, overview
additional information
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enzyme structure comparisons, overview
additional information
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inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase, the fructooligosaccharide composition containing mostly 1-kestose, 6-kestose, and neokestose obtained in the presence of different ethanol concentrations is relatively constant, while the changes in the sucrose concentration and temperature show slight changes in the ratio between 1-kestose and 6-kestose
additional information
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the catalytic triad is formed by residues Asp135, Asp309 and Glu401
additional information
the catalytic triad is formed by residues Asp135, Asp309 and Glu401
additional information
the enzyme has very high catalytic activity and stability. Key importance of residues Trp109, His113, Glu146 and Glu236 for the catalysis of Lsc3, the catalytic triad is formed by D62, D219, and E303
additional information
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the enzyme has very high catalytic activity and stability. Key importance of residues Trp109, His113, Glu146 and Glu236 for the catalysis of Lsc3, the catalytic triad is formed by D62, D219, and E303
additional information
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the recombinant enzyme shows similar biochemical properties to the native one
additional information
the three-dimensional structure of enzyme Lsc2 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
additional information
the three-dimensional structure of enzyme Lsc2 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
additional information
the three-dimensional structure of enzyme Lsc3 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
additional information
the three-dimensional structure of enzyme Lsc3 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
additional information
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the three-dimensional structure of enzyme Lsc2 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
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additional information
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the enzyme has very high catalytic activity and stability. Key importance of residues Trp109, His113, Glu146 and Glu236 for the catalysis of Lsc3, the catalytic triad is formed by D62, D219, and E303
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additional information
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the three-dimensional structure of enzyme Lsc3 of Pseudomonas syringae pv. tomato is modeled on the basis of crystal structures of either LsdA of Gluconacetobacter diazotrophicus or beta-fructofuranosidase of Arthrobacter sp. K-1
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additional information
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the recombinant enzyme shows similar biochemical properties to the native one
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additional information
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enzyme structure comparisons, overview
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additional information
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inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase, the fructooligosaccharide composition containing mostly 1-kestose, 6-kestose, and neokestose obtained in the presence of different ethanol concentrations is relatively constant, while the changes in the sucrose concentration and temperature show slight changes in the ratio between 1-kestose and 6-kestose
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