2.1.1.341: vanillate/3-O-methylgallate O-demethylase

This is an abbreviated version!
For detailed information about vanillate/3-O-methylgallate O-demethylase, go to the full flat file.

Word Map on EC 2.1.1.341

2.1.1.341

demethylation

protocatechuate

lignin

4,5-cleavage

catechol

paucimobilis

methanol

sphingomonas

4,5-dioxygenase

h4folate

sphingobium

dioxygenase

diols

Reaction

Synonyms

DesA, LigM, Mtv, syringate O-demethylase, tetrahydrofolate-dependent O-demethylase, vanillate/3-O-methylgallate O-demethylase, vanillate/3MGA O-demethylase

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.341 vanillate/3-O-methylgallate O-demethylase

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Results	in table
1	Activating Compound
3	AA Sequence
4	Cloned(Commentary)
3	Cofactor
2	Crystallization (Commentary)
6	General Information
1	Inhibitors
2	kcat/KM [mM/s]
2	Ki Value [mM]
5	KM Value [mM]
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
14	Organism
4	Pathway
4	pH Optimum
2	pH Range
12	Protein Variants
2	Reaction
9	Reference
29	Substrates and Products (Substrate)
5	Subunits
15	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.1.341 - vanillate/3-O-methylgallate O-demethylase

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Go to Protein Variants Search

H60A

Sphingobium sp.

G2IQS7

loss of catalytic activity, mutant is able to bind both substrates

740443

Y247F

Sphingobium sp.

G2IQS7

loss of catalytic activity, mutant is able to bind both substrates

740443

Y31A

Sphingobium sp.

G2IQS7

loss of catalytic activity

740443

H60A

Sphingomonas paucimobilis

Q60FX1

97-99% loss of activity

741347

M61A

Sphingomonas paucimobilis

Q60FX1

97-99% loss of activity

741347

R122A

Sphingomonas paucimobilis

Q60FX1

97-99% loss of activity

741347

R122A/R147A

Sphingomonas paucimobilis

Q60FX1

complete loss of activity

741347

Y247F

Sphingomonas paucimobilis

Q60FX1

almost complete loss of activity, no discernible effect on overall protein fold or solution behavior

741347

Y29A

Sphingomonas paucimobilis

Q60FX1

97-99% loss of activity

741347

Y31A

Sphingomonas paucimobilis

Q60FX1

97-99% loss of activity

741347

additional information

2 entries