1.8.99.B1: protein-disulfide reductase (acceptor)
This is an abbreviated version!
For detailed information about protein-disulfide reductase (acceptor), go to the full flat file.
Reaction
Synonyms
AaPDO, ApPDO, disulfide bond-forming enzyme, glutaredoxin related protein, PDO, PfPDO, protein disulfide oxidoreductase, SSO0192, SSO10610, Sso1120, SsPDO
ECTree
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Purification
Purification on EC 1.8.99.B1 - protein-disulfide reductase (acceptor)
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recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21-Codon Plus (DE3)RIL by ultracentrifugation, heat treatment at 70°C for 20 min, metal chelating affinity chromatography, dialysis, anion exchange chromatography, and again dialysis
wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S