Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes C35S, C146S and C35S/C146S in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
C146S | not active in the insulin reductase assay, oxidation of the dithiol peptide NRCSQGSCWN is reduced | Pyrococcus furiosus |
C35S | active in the insulin reductase assay, oxidation of the dithiol peptide NRCSQGSCWN is comparable to wild-type enzyme | Pyrococcus furiosus |
C35S/C146S | not active in the insulin reductase assay, no oxidation of the dithiol peptide NRCSQGSCWN | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25650 | - |
wild-type enzyme electrospray mass spectroscopy | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-dithiol + oxidized acceptor | Pyrococcus furiosus | intracellular proteins of certain hyperthermophilic archaea are rich in disulfide bonds. Disulfide bonds stabilize many thermostable proteins | protein-disulfide + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q51760 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiol peptide NRCSQGSCWN + acceptor | - |
Pyrococcus furiosus | disulfide peptide NRCSQGSCWN + reduced acceptor | - |
? | |
protein-dithiol + oxidized acceptor | intracellular proteins of certain hyperthermophilic archaea are rich in disulfide bonds. Disulfide bonds stabilize many thermostable proteins | Pyrococcus furiosus | protein-disulfide + reduced acceptor | - |
? | |
[insulin]-disulfide + reduced dithiothreitol | - |
Pyrococcus furiosus | [insulin]-dithiol + oxidized dithiothreitol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PfPDO | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus furiosus |