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1.14.19.70: mycocyclosin synthase

This is an abbreviated version!
For detailed information about mycocyclosin synthase, go to the full flat file.

Word Map on EC 1.14.19.70

Reaction

cyclo(L-tyrosyl-L-tyrosyl)
+ 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ +
O2
=
mycocyclosin
 + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O

Synonyms

CYP121, EC 1.14.21.9, rv2276

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.70 mycocyclosin synthase

Crystallization

Crystallization on EC 1.14.19.70 - mycocyclosin synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
fluconazole-bound CYP121, sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 and 0.2 M NaSCN
in complex with cyclo(L-tyrosyl-L-tyrosyl), sitting drop vapor diffusion method
ligand-bound enzyme, sitting drop vapor diffusion method, using in 100 mM Na-MES bufferat pH 5.0-5.5 and 1.6-2.2 M (NH4)2SO4, at 6°C
native enzyme or in complex with iodopyrazole, PtCN, K2PtCl4, or Hg(NO3)2, vapor diffusion method, using 30% (w/v) polyethylene glycol 8000, 0.2 M ammonium sulfate, pH 6.5