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1.14.14.158: carotenoid epsilon hydroxylase

This is an abbreviated version!
For detailed information about carotenoid epsilon hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.158

Reaction

alpha-carotene
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
alpha-cryptoxanthin
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

carotenoid epsilon hydroxylase, carotenoid epsilon-hydroxylase, carotenoid epsilon-ring hydroxylase, CHXE, CitCYP97C, CYP97a3, CYP97C, CYP97C1, CYP97C19, EC 1.14.99.45, epsilon carotene hydroxylase, epsilon ring hydroxylase, epsilon-ring carotene hydroxylase, epsilon-ring hydroxylase, heme-containing cytochrome P450 carotene epsilon-ring hydroxylase, IdCHXE, LUT1, LUT1/CYP97C1, LUT5, LUT5/CYP97A3, zeinoxanthin 3'-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.158 carotenoid epsilon hydroxylase

Cloned

Cloned on EC 1.14.14.158 - carotenoid epsilon hydroxylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene
gene CHXE, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, quantitative real-time PCR enzyme expression analysis
-
gene CitCYP97C, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis. When CitHYb and CitCYP97C are coexpressed in Escherichia coli strain BL21(DE3) the monohydroxylated zeinoxanthin, which is produced by CitHYb, is further converted to lutein by CitCYP97C, when CitCYP97A and CitCYP97C are coexpressed or CitCYP97B and CitCYP97C are coexpressed, no hydroxylated carotene is detected in the alpha-carotene- and beta-carotene-accumulating Escherichia coli strain BL21(DE3) cells
gene CYP97C19, cDNA is cloned from leaves, the ZmCYP97C19 gene has nine introns and ten exons, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure, overview. Functional recombinant expression under the control of constitutive CaMV 35S promoter in rosettes leaves of the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. Lutein accumulates to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants, carotenoid extraction and quantification from Arabidopsis thaliana lut1-CYP97C1 leaves
gene LeLUT1, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, expression of gene LeLUT1-GFP fusion protein in chloroplasts of Arabidopsis thaliana mesophyll protoplasts, quantitative real-time PCR expression analysis
BT012891
genes lut1 and lut5, real-time PCR expression analysis