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1.13.11.87: endo-cleaving rubber dioxygenase

This is an abbreviated version!
For detailed information about endo-cleaving rubber dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.87

Reaction

Cleavage of cis-1,4-polyisoprene polymers into a mixture of compounds, including a C20 compound ((4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal), a C25 compound ((4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal), a C30 compound ((4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal), and larger isoprenologes such as C35, C40, C45, and higher analogues =

Synonyms

GPOL_174p00150, GPOL_c48310, latex clearing protein, latex-clearing protein, Lcp, Lcp protein, Lcp1, lcp1VH2, Lcp2, lcp2VH2, LcpK30, polyisoprene-cleaving rubber oxygenase, roxA, RoxB, rubber oxidizing protein, rubber oxygenase, rubber oxygenase B

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.87 endo-cleaving rubber dioxygenase

Cloned

Cloned on EC 1.13.11.87 - endo-cleaving rubber dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a DELTAroxA35Y/DELTAroxB35Y background of Steroidobacter cummioxidans 35Y
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expressed in Escherichia coli C41 cells
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expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli JM109 cells
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expression in Escherichia coli
expression of Lcp of Streptomyces sp. K30 in a DELTAroxA background of Xanthomonas sp. strain 35Y
extression in Streptomyces lividans TK23
heterologous expression of the NVL3 lcp in Escherichia coli BL21(DE3)
when Lcp from Streptomyces sp. strain K30 is heterologously expressed in strains TK23 and TK24 of Streptomyces lividans and a strain of Saccharopolyspora erythraea with plasmid pIJ6021::lcp, the recombinant strains acquire the ability to cleave synthetic poly(cis-1,4-isoprene)