EC Number   |
Protein Variants |
Reference |
|---|
    2.1.1.37 | A34V/K44Q/M66T/L214S/Y229H |
isolation of a relaxed-specificity mutant of the enzyme also methylates, at a lower rate, GG(G/C)CC sites |
704283 |
| 2.1.1.37 | A609T |
naturally occuring mutation causing recessive genetic disorder, ICF syndrome, the mutation does not affect the association of Dnmt3b with Mbd4 or Tdg |
705118 |
| 2.1.1.37 | C141S |
site-directed mutagenesis in motif IV |
702450 |
| 2.1.1.37 | C368A |
the mutation does not affect the enzyme activity |
702450 |
| 2.1.1.37 | C667G |
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity |
702212 |
| 2.1.1.37 | C667G/C670G |
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity |
702212 |
| 2.1.1.37 | C670G |
zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity |
702212 |
| 2.1.1.37 | D73A |
25fold improvement in affinity to S-adenosyl-L-methionine |
659530 |
| 2.1.1.37 | DELAT1-501 |
to accelerate the rate of DNMT1 with the hemimethylated substrate, the 501 amino acids at the N terminus are deleted. Truncated DNMT1 exhibits a 4fold increased kcat/Km as compared with the full-length enzyme and does not require allosteric activation by hemimethylated DNA |
718565 |
| 2.1.1.37 | DELTA1-501 |
no stimulation by methylated DNA |
657995 |
