Cloned (Comment) | Organism |
---|---|
DNMT1, overexpression of HA-tagged CXXC deletion DNMT1 mutant, expression of wild-type and deletion mutant enzymes in COS-7 cells and Escherichia coli, CXXC deleted DNMT1, mutant DNMT1DELTACXXC, is localized on replication foci | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C667G | zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity | Homo sapiens |
C667G/C670G | zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity | Homo sapiens |
C670G | zinc-finger-domain point mutation in the CXXC motif abolishes unmethylated DNA binding activity | Homo sapiens |
additional information | construction of zinc-finger-domain point mutant and deletion mutant DNMT1 clones, CXXC deleted DNMT1, i.e. mutant DNMT1DELTACXXC and DNMT1 lacking the first 580 amino acids, i.e. DNMT1DELTA580. The latter prefers binding to unmethylated DNA instead of hemimethylated or fully methylated DNA like the wild-type enzyme. Deletion of CXXC domain in DNMT1 does not affect replication foci occupancy during DNA replication. A permanent cell line with DNMT1DELTACXXC displays partial loss of genomic methylation on rDNA loci, despite the presence of endogenous wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Homo sapiens | DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P26358 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases | Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | poly(dI-dC) substrate DNA. The enzyme contains the zinc-finger CXXC domain, a zinc-coordinating domain that selectively binds unmethylated DNA and is crucial for enzymatic activity | Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the conserved CXXC domain encompassing the amino terminus region of DNMT1 cooperates with the catalytic domain for DNA methyltransferase activity, and binds to unmethylated CpG | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DNA cytosine-5 methyltransferase 1 | - |
Homo sapiens |
Dnmt1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |