EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.13.12.7 | -999 |
- |
10 min at 50°C decreases activity by 90% |
484872 |
| 1.13.12.7 | -999 |
- |
a chimeric protein derived from Photinus pyralis and Luciola cruciata luciferases is more stable than the wild type enzyme, retaining 75% of the activity after 10 min at 50°C |
484870 |
| 1.13.12.7 | -999 |
- |
higher stability in frozen state than in refrigerated |
484836 |
| 1.13.12.7 | -999 |
- |
no activity at 40°C |
698315 |
| 1.13.12.7 | -999 |
- |
thermal stability and activity at 35°C of the enzyme in presence of magnetic magnetite nanoparticles supported ionic liquids is increased compared to the free enzyme, overview |
744062 |
| 1.13.12.7 | 15 |
40 |
at 35°C native luciferase completely inactive, mutant H245N still 80% residual activity, mutant S284T and H431Y about 20% residual activity |
696089 |
| 1.13.12.7 | 15 |
45 |
when native and mutants are incubated 5 min at temperatures ranging from 15 to 45°C, D474K mutant is inactivated faster than native and D476N at all temperatures |
727075 |
| 1.13.12.7 | 16.7 |
- |
Tm value of recombinant wild-type LUC |
746213 |
| 1.13.12.7 | 20 |
- |
after 20 min only 5% activity in the absence of ionic liquids but less than 1% in the presence of [1,1,3,3-tetramethylguanidine][lactate] and [1,1,3,3-tetramethylguanidine][propionate] |
728433 |
| 1.13.12.7 | 23 |
- |
stability remains constant upon 120 min |
727631 |
