Application | Comment | Organism |
---|---|---|
biotechnology | imaging technology | Lampyris turkestanicus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Lampyris turkestanicus |
Protein Variants | Comment | Organism |
---|---|---|
H245N | red- and green-emitting luciferase, increase in thermostability, about 25% decrease in relative activity | Lampyris turkestanicus |
H431Y | red- and green-emitting luciferase, about 85% decrease in relative acitivity | Lampyris turkestanicus |
S284T | red-emitting luciferase, about 75% decrease in relative activity | Lampyris turkestanicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01612 | - |
D-luciferin | wild-type luciferase | Lampyris turkestanicus | |
0.023 | - |
D-luciferin | mutant H245N | Lampyris turkestanicus | |
0.026 | - |
D-luciferin | mutant H431Y | Lampyris turkestanicus | |
0.03 | - |
D-luciferin | mutant S284T | Lampyris turkestanicus | |
0.135 | - |
ATP | wild-type luciferase | Lampyris turkestanicus | |
0.168 | - |
ATP | mutant H245N | Lampyris turkestanicus | |
0.1872 | - |
ATP | mutant H431Y | Lampyris turkestanicus | |
0.2484 | - |
ATP | mutant S284T | Lampyris turkestanicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lampyris turkestanicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lampyris turkestanicus | Q5UFR2 | wild-type emits green light | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose | Lampyris turkestanicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
11500000000000 relative light units/mg, mutant H245N | Lampyris turkestanicus |
additional information | - |
15000000000000 relative light units/mg, wild-type luciferase | Lampyris turkestanicus |
additional information | - |
2500000000000 relative light units/mg, mutant H431Y | Lampyris turkestanicus |
additional information | - |
3600000000000 relative light units/mg, mutant S284T | Lampyris turkestanicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + ATP + O2 | assay at 25°C, pH 7.8 | Lampyris turkestanicus | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
firefly luciferase | - |
Lampyris turkestanicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
wild-type luciferase, mutant H245N and H431Y | Lampyris turkestanicus |
25 | - |
assay at | Lampyris turkestanicus |
30 | - |
mutant S284T | Lampyris turkestanicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 40 | at 35°C native luciferase completely inactive, mutant H245N still 80% residual activity, mutant S284T and H431Y about 20% residual activity | Lampyris turkestanicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Lampyris turkestanicus |
8 | - |
wild-type luciferase, mutant S284T | Lampyris turkestanicus |
8.5 | - |
mutant H245N and H431Y | Lampyris turkestanicus |