EC Number |
---|
1.21.99.3 | - |
1.21.99.3 | DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells |
1.21.99.3 | expressed in Escherichia coli Rosetta2 cells |
1.21.99.3 | expressed in Xenopus laevis oocytes |
1.21.99.3 | expression in oocytes from Xenopus laevis |
1.21.99.3 | expression of RNAi to knock down D3 in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes |
1.21.99.3 | expression of RNAi to knock down type 3 deiodinase in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes |
1.21.99.3 | isozyme type III, expression of wild-type and active site mutants in COS cells, 50fold higher expression level for the mutants than for the wild-type enzyme |
1.21.99.3 | isozyme type III, via RT-PCR from cerebellum mRNA, expression in bacteria |
1.21.99.3 | screening of neonatal skin cDNA library, DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, functional translation in Xenopus laevis oocytes, catalytis properties of recombinant and native enzyme are identical |