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1.14.13.225
medicine
elevated MICAL1 gene expression is observed in invasive breast cancer samples from human patients relative to normal tissue, while MICAL1 amplification or point mutations are associated with reduced progression free survival
764398
1.14.13.225
synthesis
protocol for obtaining high levels of recombinant protein for the redox only portion of Mical. Cold adapted chaperonins alone do not work well for expressing the Mical redox domain, while the use and removal of solubility tags destabilizes. Low-temperature expression, chaperonins and no solubility tag lead to enhanced expression
764919
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