EC Number   |
Application |
Reference |
|---|
    1.13.12.7 | analysis |
an engineered, commercial thermostable luciferase is suitable for real-time monitoring of ATP release by bacteria, both in broth culture and on agar surfaces, which allows for the estimation of viable cell number by relating luminescence onset time to initial cell concentration. The method is able to rapidly detect the effect of antibiotics on bacterial cultures |
765660 |
| 1.13.12.7 | analysis |
analytical assay of metabolites like ATP, CoA, pyrophosphate, AMP |
698393 |
| 1.13.12.7 | analysis |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
746376 |
| 1.13.12.7 | analysis |
development of a quantitative and highly sensitive luciferase-based assay for bacterial toxins, overview |
675322 |
| 1.13.12.7 | analysis |
expression of bioluminescent luciferase can be used for rapid and high throughput screening of drugs, e.g. quinolines, acting on Leishmania amastigote-harbouring macrophages and for quantitative real-time monitoring of parasitism features in living mice, overview |
672992 |
| 1.13.12.7 | analysis |
luciferase-based determination of ATP/NAD(H) pools in combination with a microplate reader and the marine model bacterium Phaeobacter inhibens. Grey multiwell plates best balance sensitivity and crosstalk, and optimal incubation times are 5 min and 30 min for the ATP and NAD(H) assay, respectively, together allowing limits of detection of 0.042, 0.470 and 0.710 nM for ATP, NAD+, and NADH, respectively |
-, 765353 |
| 1.13.12.7 | analysis |
luminescence-based assays for ATP measurement in clinical chemistry and hygiene monitoring |
484875 |
| 1.13.12.7 | analysis |
optical bioluminescence-based molecular imaging |
697750 |
| 1.13.12.7 | analysis |
the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice |
744013 |
| 1.13.12.7 | biotechnology |
extensive and advantageous application of this enzyme in biotechnology is restricted due to its low thermal stability |
765646 |
