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Literature summary for 1.13.12.7 extracted from

  • Ihssen, J.; Jovanovic, N.; Sirec, T.; Spitz, U.
    Real-time monitoring of extracellular ATP in bacterial cultures using thermostable luciferase (2021), PLoS ONE, 16, e0244200 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
analysis an engineered, commercial thermostable luciferase is suitable for real-time monitoring of ATP release by bacteria, both in broth culture and on agar surfaces, which allows for the estimation of viable cell number by relating luminescence onset time to initial cell concentration. The method is able to rapidly detect the effect of antibiotics on bacterial cultures Photuris pensylvanica

Organism

Organism UniProt Comment Textmining
Photuris pensylvanica Q27757
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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 Photuris pensylvanica
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Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 4 h, 90% resiudal activity, 24 h, 70% residual activity, respectively, for engineered commercial enzyme. Wild-type enzyme loses 80% of initial activity within 39 min Photuris pensylvanica