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Literature summary for 2.1.1.B75 extracted from

  • Cui, H.; Song, M.; Ban, Y.; Jun, S.; Kwon, A.; Lee, J.; Yoon, Y.
    High-yield production of multiple O-methylated phenylpropanoids by the engineered Escherichia coli-Streptomyces cocultivation system (2019), Microb. Cell Fact., 18, 67 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B75

Application

Application Comment Organism
synthesis OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant Streptomyces avermitilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces venezuelae Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q79ZE8
-
-
Streptomyces avermitilis DSM 46492 Q79ZE8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + apigenin
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
 ?
S-adenosyl-L-methionine + apigenin
-
 Streptomyces avermitilis DSM 46492 S-adenosyl-L-homocysteine + genkwanin + 5-hydroxy-7,4'-dimethoxyflavone
-
 ?
S-adenosyl-L-methionine + naringenin
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
 ?
S-adenosyl-L-methionine + naringenin
-
 Streptomyces avermitilis DSM 46492 S-adenosyl-L-homocysteine + sakuranetin + 5-hydroxy-7,4'-dimethoxyflavanone + 4'-hydroxy-5,7-dimethoxyflavanone
-
 ?
S-adenosyl-L-methionine + resveratrol
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
 ?
S-adenosyl-L-methionine + resveratrol
-
 Streptomyces avermitilis DSM 46492 S-adenosyl-L-homocysteine + desoxyrhapontigenin + 5-hydroxy-3,4'-dimethoxystilbene
-
 ?

Synonyms

Synonyms Comment Organism
OMT2
-
 Streptomyces avermitilis
SAVERM_2382
-
 Streptomyces avermitilis

General Information

General Information Comment Organism
physiological function OMT2 exhibits more diverse regiospecificity and catalyzes mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it is expressed in Streptomyces venezuelae compared to expression in Escherichia coli. For the efficient production of multi-methylated phenylpropanoids, a cocultivation system uses engineered Escherichia coli strains producing pterostilbene, naringenin, and apigenin, respectively, along with a OMT2-expressing Streptomyces venezuelae mutant Streptomyces avermitilis