Literature summary for 2.1.1.226 extracted from

Monshupanee, T.; Johansen, S.K.; Dahlberg, A.E.; Douthwaite, S.
Capreomycin susceptibility is increased by TlyA-directed 2-O-methylation on both ribosomal subunits (2012), Mol. Microbiol., 85, 1194-1203.

Search for more literature for 2.1.1.226

Cloned(Commentary)

Cloned (Comment)	Organism
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Thermus thermophilus
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Geobacillus stearothermophilus
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Mycobacterium tuberculosis
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Streptomyces coelicolor
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Mycolicibacterium smegmatis
gene tlyA, DNA and amino acid sequence determination, analysis and comparison, expression in Escherichia coli strain DH1	Brachyspira hyodysenteriae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermus thermophilus
Mg2+	required	Geobacillus stearothermophilus
Mg2+	required	Mycobacterium tuberculosis
Mg2+	required	Streptomyces coelicolor
Mg2+	required	Mycolicibacterium smegmatis
Mg2+	required	Brachyspira hyodysenteriae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Geobacillus stearothermophilus	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Mycobacterium tuberculosis	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Streptomyces coelicolor	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Mycolicibacterium smegmatis	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Thermus thermophilus	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Brachyspira hyodysenteriae	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Brachyspira hyodysenteriae	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Mycobacterium tuberculosis H37Rv	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Mycolicibacterium smegmatis mc(2)155	-	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Brachyspira hyodysenteriae	Q06803	gene tlyA	-
Geobacillus stearothermophilus	-	gene tlyA	-
Mycobacterium tuberculosis	-	gene tlyA	-
Mycobacterium tuberculosis H37Rv	-	gene tlyA	-
Mycolicibacterium smegmatis	A0QYR0	gene tlyA	-
Mycolicibacterium smegmatis mc(2)155	A0QYR0	gene tlyA	-
no activity in Escherichia coli	-	-	-
Streptomyces coelicolor	-	gene tlyA	-
Thermus thermophilus	-	gene tlyA	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	-	gene tlyA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the TlyAII enzyme methylates efficiently at C1920 in 50S subunits in vitro, although weaker activity is also detected on 70S ribosome and 23S rRNA substrates	Mycolicibacterium smegmatis	?	-	?
additional information	the TlyAII enzyme methylates efficiently at C1920 in 50S subunits in vitro, although weaker activity is also detected on 70S ribosome and 23S rRNA substrates	Mycolicibacterium smegmatis mc(2)155	?	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Geobacillus stearothermophilus	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Mycobacterium tuberculosis	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Streptomyces coelicolor	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Mycolicibacterium smegmatis	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	Thermus thermophilus	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	Brachyspira hyodysenteriae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Mycobacterium tuberculosis H37Rv	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Mycolicibacterium smegmatis mc(2)155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	-	Brachyspira hyodysenteriae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 23S rRNA	the 50S subunit is the preferred substrate, a modest amount of methylation is seen with free 23S rRNA and 70S ribosomes	Brachyspira hyodysenteriae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 50S rRNA	-	Mycolicibacterium smegmatis	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 50S rRNA	-	Brachyspira hyodysenteriae	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?
S-adenosyl-L-methionine + cytidine1920 in 50S rRNA	-	Mycolicibacterium smegmatis mc(2)155	S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 50S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
TlyA	-	Thermus thermophilus
TlyA	-	Geobacillus stearothermophilus
TlyA	-	Mycobacterium tuberculosis
TlyA	-	Streptomyces coelicolor
TlyA	-	Mycolicibacterium smegmatis
TlyA	-	Brachyspira hyodysenteriae
TlyAI	-	Thermus thermophilus
TlyAII	-	Geobacillus stearothermophilus
TlyAII	-	Mycobacterium tuberculosis
TlyAII	-	Streptomyces coelicolor
TlyAII	-	Mycolicibacterium smegmatis
TlyAII	-	Brachyspira hyodysenteriae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Thermus thermophilus
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Geobacillus stearothermophilus
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Mycobacterium tuberculosis
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Streptomyces coelicolor
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Mycolicibacterium smegmatis
37	-	assay at with Mycobacterium smegmatis ribosomal substrates	Brachyspira hyodysenteriae
50	-	assay at with Thermus thermophilus ribosomal substrates	Thermus thermophilus
50	-	assay at with Thermus thermophilus ribosomal substrates	Geobacillus stearothermophilus
50	-	assay at with Thermus thermophilus ribosomal substrates	Mycobacterium tuberculosis
50	-	assay at with Thermus thermophilus ribosomal substrates	Streptomyces coelicolor
50	-	assay at with Thermus thermophilus ribosomal substrates	Mycolicibacterium smegmatis
50	-	assay at with Thermus thermophilus ribosomal substrates	Brachyspira hyodysenteriae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thermus thermophilus
7.5	-	assay at	Geobacillus stearothermophilus
7.5	-	assay at	Mycobacterium tuberculosis
7.5	-	assay at	Streptomyces coelicolor
7.5	-	assay at	Mycolicibacterium smegmatis
7.5	-	assay at	Brachyspira hyodysenteriae

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Thermus thermophilus
S-adenosyl-L-methionine	-	Geobacillus stearothermophilus
S-adenosyl-L-methionine	-	Mycobacterium tuberculosis
S-adenosyl-L-methionine	-	Streptomyces coelicolor
S-adenosyl-L-methionine	-	Mycolicibacterium smegmatis
S-adenosyl-L-methionine	-	Brachyspira hyodysenteriae

General Information

General Information	Comment	Organism
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Thermus thermophilus
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Geobacillus stearothermophilus
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Mycobacterium tuberculosis
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Streptomyces coelicolor
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Mycolicibacterium smegmatis
evolution	TlyA orthologues occur in diverse bacteria and fall into two distinct groups. One group, termed TlyAI, has shorter N- and C-termini and methylates only C1920. The second group, TlyAII, includes the mycobacterial enzyme, and these longer orthologues methylate at both C1409 (c.f. EC 2.1.1.227), and C1920	Brachyspira hyodysenteriae
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Thermus thermophilus
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Geobacillus stearothermophilus
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Mycobacterium tuberculosis
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Streptomyces coelicolor
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Mycolicibacterium smegmatis
malfunction	inactivation of TlyA and loss of its activity confer resistance to capreomycin and viomycin	Brachyspira hyodysenteriae
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Thermus thermophilus
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Geobacillus stearothermophilus
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Mycobacterium tuberculosis
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Streptomyces coelicolor
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Mycolicibacterium smegmatis
additional information	the cyclic peptide antibiotics capreomycin and viomycin bind on the ribosomal S70 subunit interface close to nucleotides C1409 in 16S rRNA and C1920 in 23S rRNA	Brachyspira hyodysenteriae

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Release 2023.1 (January 2023)