Cloned (Comment) | Organism |
---|---|
PCR amplification, expression in Escherichia coli BL21 (DE3) | Chromohalobacter sp. HS-2 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 10% is optimal NaCl concentration for Chromohalobacter sp. strain HS-2 | Chromohalobacter sp. HS-2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + [reduced NADPH-hemoprotein reductase] + O2 | Chromohalobacter sp. HS-2 | - |
2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? | |
benzoate + [reduced NADPH-hemoprotein reductase] + O2 | Chromohalobacter sp. HS2 | - |
2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromohalobacter sp. HS-2 | A8I4E8 | ben A subunits; isolated from salted fermented clams | - |
Chromohalobacter sp. HS-2 | A8I4F1 | ben B subunits; isolated from salted fermented clams | - |
Chromohalobacter sp. HS-2 | A8I4F4 | ben C subunits; isolated from salted fermented clams | - |
Chromohalobacter sp. HS2 | A8I4E8 | ben A subunits; isolated from salted fermented clams | - |
Chromohalobacter sp. HS2 | A8I4F1 | ben B subunits; isolated from salted fermented clams | - |
Chromohalobacter sp. HS2 | A8I4F4 | ben C subunits; isolated from salted fermented clams | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chromohalobacter sp. HS-2 | 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? | |
benzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chromohalobacter sp. HS2 | 2-hydro-1,2-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | cis-benzoate dihydrodiol is subsequently dehydrogenated to catechol which is cleaved further | ? |
Synonyms | Comment | Organism |
---|---|---|
benzoate 1,2-dioxygenase | product of genes benA (large subunit), benB (small subunit), benC (electron transfer component) | Chromohalobacter sp. HS-2 |