Application | Comment | Organism |
---|---|---|
analysis | development of a single-quantum-dot-based fluorescence resonance energy transfer (FRET) sensor for the identification of specific FTO demethylase inhibitors. The FTO-mediated demethylation of m6A can induce the cleavage of demethylated DNA to generate the biotinylated DNA fragments, which may function as capture probes to assemble the Cy5-labeled reporter probes onto the quantum dot surface, enabling the occurrence of FRET between the quantum dot and Cy5 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diacerein | not inhibitiory to isoform ALKBH5 | Homo sapiens | |
additional information | not inhibitiory: rhein, diacerein | Homo sapiens | |
Plumbagin | - |
Homo sapiens | |
Rhein | not inhibitiory to isoform ALKBH5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P6C2 | - |
- |
Homo sapiens | Q9C0B1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biotin-AAGCTCCCATGTTAGGm6ATCAGTGTCTCGAG-biotin + 2-oxoglutarate + O2 | - |
Homo sapiens | biotin-AAGCTCCCATGTTAGGATCAGTGTCTCGAG-biotin + formaldehyde + succinate + CO2 | - |
? | |
CATGTTAGGATCAGTG + 2-oxoglutarate + O2 | - |
Homo sapiens | CATGTTAGGm6ATCAGTG + formaldehyde + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fat mass and obesity-associated enzyme | - |
Homo sapiens |
FTO | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00109 | - |
pH 7.5, 30°C | Homo sapiens | Plumbagin | |
0.00151 | - |
pH 7.5, 30°C | Homo sapiens | diacerein | |
0.00163 | - |
pH 7.5, 30°C | Homo sapiens | Plumbagin | |
0.00166 | - |
pH 7.5, 30°C | Homo sapiens | Rhein |