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2.4.1.82: galactinol-sucrose galactosyltransferase

This is an abbreviated version!
For detailed information about galactinol-sucrose galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.82

Reaction

alpha-D-galactosyl-(1->3)-1D-myo-inositol
+
sucrose
=
myo-inositol
+
raffinose

Synonyms

alkaline alpha galactosidase 1, At4g01970, galactinol:sucrose 6-galactosyl transferase, galactosyltransferase, galactinol-sucrose, More, raffinose synthase, raffinose synthase 1, raffinose synthase 2, raffinose synthase 3, raffinose synthase 4, raffinose synthase 5, raffinose synthase 6, RafS, RFO synthase/galactosylhydrolase, RFS4, RFS5, RS1, RS2, RS3, RS4, RS5, RS6, ZmRAFS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.82 galactinol-sucrose galactosyltransferase

Application

Application on EC 2.4.1.82 - galactinol-sucrose galactosyltransferase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
synthesis
use of an in vitro multienzyme system using sucrose synthase (SUS), UDP-glucose 4-epimerase (GalE), galactinol synthase (GS), raffinose synthase (RS), and stachyose synthase (STS) and intermedia UDP and inositol, which can be recycled, to synthesize raffinose,. The reaction system produces 11.1mM raffinose using purified enzymes under optimal reaction conditions and substrate concentrations. Optimization further increases raffinose production to 86.6 mM and enables the synthesis of 61.1 mM stachyose. The UDP turnover number reaches 337. Inositol in the reaction system is recycled five times, and 255.8 mM raffinose (128.9 g/liter) is obtained