2.4.1.339: beta-1,2-mannobiose phosphorylase

This is an abbreviated version!
For detailed information about beta-1,2-mannobiose phosphorylase, go to the full flat file.

Reaction

Synonyms

Lin0857

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.339 beta-1,2-mannobiose phosphorylase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
3	AA Sequence
1	Cloned(Commentary)
1	Crystallization (Commentary)
6	kcat/KM [mM/s]
8	KM Value [mM]
2	Molecular Weight [Da]
3	Organism
16	PDB ID
2	pH Optimum
2	pH Range
1	pH Stability
1	Purification (Commentary)
1	Reaction
3	Reference
7	Substrates and Products (Substrate)
1	Subunits
1	Synonyms
1	Systematic Name
1	Temperature Stability [°C]
6	Turnover Number [1/s]

Crystallization

print visible entries

print all entries

Crystallization on EC 2.4.1.339 - beta-1,2-mannobiose phosphorylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Crystallization (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

sitting drop vapor diffusion method at 20°C, crystals complexed with beta-1,2-Man2 and beta-1,2-Man3 are prepared by soaking in the cryoprotectant solution supplemented with 50 mM beta-1,2-Man2 or 10 mM beta-1,2-Man3. beta-1,2-Mannotriose is bound in a U-shape, interacting with a phosphate analog at both ends. Lin0857 has a unique dimer structure connected by a loop, and a significant open–close loop displacement iss observed for substrate entry. A long loop, which is exclusively present in Lin0857, covers the active site to limit the pocket size. A structural basis for substrate recognition and phosphorolysis is provided

Listeria innocua

Q92DF6

738245