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2.4.1.248: cycloisomaltooligosaccharide glucanotransferase

This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.248

Reaction

cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =

Synonyms

cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.248 cycloisomaltooligosaccharide glucanotransferase

Cloned

Cloned on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
WP_028992696
expression in Escherichia coli
gene cit, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
gene cit, expression in Escherichia coli
overexpression of CITase in Bacillus circulans strain G22-10, and, with the alpha-amylase promoter (PamyQ) and amyQ signal sequence of Bacillus amyloliquefaciens, in a protease-deficient Bacillus subtilis strain 168 as extracellular protein. The Bacillus subtilis host-vector system allows production of cycloisomaltooligosaccharides by direct fermentation of dextran along with high CITase production, which is not possible in Bacillus circulans G22-10 due to growth inhibition by dextran at high concentrations and limited production of CITase, stable expression up to 48 h
the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified
WP_028992696