2.4.1.21: starch synthase (glycosyl-transferring)

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For detailed information about starch synthase (glycosyl-transferring), go to the full flat file.

Reaction

Synonyms

adenosine diphosphate glucose-starch glucosyltransferase, adenosine diphosphoglucose-starch glucosyltransferase, ADP-glucose starch synthase, ADP-glucose synthase, ADP-glucose transglucosylase, ADP-glucose-starch glucosyltransferase, ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase, ADPG starch synthetase, ADPG-starch glucosyltransferase, AtSS2, AtSSII, AtSSIII, CkGBSS, dull1, GBSS, GBSSI, glgA, glucosyltransferase, adenosine diphosphoglucose-starch, glycogen synthase, glycogen/starch synthase, granule bound starch synthase, granule-bound starch synthase, granule-bound starch synthase I, GSase, OsGBSSII, OsSSIII-1, OsSSIII-2, OsSSIV-1, OsSSIV-2, PvSSI, PvSSII-1, SBD123, SS1, SS4, SSI, SSII, SSII(a), SSIIa, SSIIc, SSIII, SSIII(a), SSIII-1, SSIII-B, SSIII-C, SSIIIa, SSIIIb, SSIV, SSS, SSSI, SSV, starch synthase, starch synthase 1, starch synthase 4, starch synthase I, starch synthase II, starch synthase IIa, starch synthase III, starch synthase III-1, starch synthase III-2, starch synthase III-B, starch synthase IIIa, starch synthase IV, starch synthase IV-1, starch synthase IV-2, starch synthetase, starch-synthase III, starch/glycogen synthase, TaSSIVb, TVAG_258220

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.21 starch synthase (glycosyl-transferring)

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Results	in table
36	Activating Compound
22499	AA Sequence
7	Application
1	CAS Registry Number
45	Cloned(Commentary)
5	Crystallization (Commentary)
6	Expression
21	General Information
42	Inhibitors
2	Ki Value [mM]
96	KM Value [mM]
53	Localization
18	Metals/Ions
28	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
207	Organism
7	Pathway
26	PDB ID
12	pH Optimum
2	pH Range
1	pH Stability
1	pI Value
4	Posttranslational Modification
36	Protein Variants
44	Purification (Commentary)
1	Reaction
1	Reaction Type
146	Reference
83	Source Tissue
22	Specific Activity [micromol/min/mg]
12	Storage Stability
213	Substrates and Products (Substrate)
38	Subunits
188	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
18	Temperature Stability [°C]
4	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.4.1.21 - starch synthase (glycosyl-transferring)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, fraction V, unstable

Zea mays subsp. mays

-

488919

-15°C, fraction IV, 6 months

Zea mays subsp. mays

-

488919

-20°C, 6 months, 50% loss of activity

Sorghum bicolor

-

488898

-20°C, at least 4 months, no loss of activity

Solanum tuberosum

-

488892

-70°C, 50 mM Tris/acetate buffer, pH 7.5, 2.5 mM DTT, 10 mM EDTA, 5% w/v sucrose

Zea mays

-

488897

-80°C, purified His-tagged enzyme, in 20 mM triethanolamine-HCl, pH 8.0, and 20% v/v glycerol, stable for at least 6 months

Streptomyces coelicolor

-

719739

-85°C, at least 1 year

Escherichia coli

-

488910

0-4°C, ammonium sulfate precipitate

Oryza sativa

-

488915

0°C, 1 month, 30% loss of activity

Sorghum bicolor

-

488898

4°C, at least 2 weeks, no loss of activity

Solanum tuberosum

-

488892

addition of 0.04% (w/v) polyvinyl alcohol 50 K and 1 mM dithiothreitol to the glycine buffer, pH 8.4, leads to long-term stability and

Solanum tuberosum

-

719220

higher yields of both starch synthase and starch branching enzyme, due to activation of inactive enzymes

Solanum tuberosum

-

719220